Characteristics of Patients Hospitalized with 2009 H1N1 Influenza in a Tertiary Care Hospital in Southern Saudi Arabia

Adnan Agha1, Abdulqader Alrawi2, Cesar V. Munayco3, Mohammed S. Al Ayed4, Mohammad Al-Hakami1, Hassan Korairi2 and Abdelhaleem Bella5

1 Department of Internal Medicine, Armed Forces Hospital Southern Region, Khamis Mushyt, Saudi Arabia
Department of Preventive Medicine, Armed Forces Hospital Southern Region, Khamis Mushyt, Saudi Arabia
Direccin General de Epidemiologa, Ministry of Health of Peru
Department of Pediatrics, College Of Medicine, Najran University, Najran, Saudi Arabia
Department of Internal Medicine, University of Dammam, Saudi Arabia

Correspondence to: Dr. Adnan Agha. Registrar, Department of Internal Medicine, Armed Forces Hospital Southern Region, PO Box 101 Khamis Mushyt, Kingdom of Saudi Arabia. E-mail:

Published: January 16, 2012
Received: October 2, 2011
Accepted: December 15, 2011
Mediterr J Hematol Infect Dis 2012, 4(1): e2012002 , DOI 10.4084/MJHID.2012.002
This article is available on PDF format at:
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Background: Pandemic influenza A (H1N1) virus emerged and spread globally in the spring of 2009. We describe the clinical features of the patients who were hospitalized with 2009 H1N1 influenza July 2009 to June 2010 in a tertiary care hospital in Khamis Mushyt, Saudi Arabia. We analyzed the clinical and laboratory variables in order to determine predictors of poor outcome.
Methods: We performed a prospective study in all patients who were hospitalized for at least 48 hours and with a positive test for 2009 H1N1 virus through RT-PCR(real time polymerase chain reaction). Their epidemiological, clinical, biochemical characteristics were collected and the hospital course of the patients with eventual outcome (discharge or death) was observed. We applied a logistic regression analysis to determine the best predictor of death.
Results: A total of 52 patients (15 males) were adults and 65 were pediatrics (< 12 years of age) (19 males). The common presenting signs and/or symptoms associated with the disease was fever >38.5 C (n=85; 72.6%), dry cough (n=81; 69.2%), dyspnea (n=40; 34.5%), tachycardia (n=96; 83.5%) and saturation less than 90% in room air on pulse oximetry (n=65; 55.6%). The complications included pneumonia (40.2 %), intensive care unit admission (19.2%) and death (16.7%).
Conclusions: We found that hypoxia at admission was the most important predictive factor of poor outcome (death) with area under curve of 0.768.

During the spring of 2009, a novel influenza A (H1N1) virus of swine origin caused human infection and acute respiratory illness in Mexico.[1,2] After initially spreading across the United States and Canada,[3] the virus spread globally, resulting in the first influenza pandemic since 1968 with circulation outside the usual influenza season in the Northern Hemisphere.[4] As of March 2010, almost all countries had reported cases, and more than 17,700 deaths among laboratory-confirmed cases had been reported to the World Health Organization (WHO), which significantly underestimates the pandemic’s impact.[5] In the United States alone, an estimated 59 million illnesses, 265,000 hospitalizations, and 12,000 deaths had been caused by the 2009 H1N1 virus as of February 2010, while in April 2010 WHO announced that H1N1 had entered a post-pandemic phase.[6]
The original Pandemic 2009 H1N1 virus genome actually derives six genes from triple-reassortant North American swine virus lineages and two genes from Eurasian swine virus lineages.[7] The first case of pandemic influenza A (H1N1) virus was reported in Saudi Arabia on June 3, 2009.[8] On September 12, 2009, the Saudi Ministry of Health issued a national plan for the management of the flu-like pandemics, specifically pandemic influenza A (H1N1) virus infections.[9] Although there have been various risk factors identified in recent literature from Saudi Arabia for poor outcome among patient with 2009 H1N1 infection, like delay in initiation of antiviral therapy, young age female sex and patients with chest pain, confusion or loss of consciousness;[10] but to-date there has been no study done to identify a model that could predict risk factor for poor outcome in these patients.
Here we attempt to describe the clinical and biochemical features of the patients who were hospitalized with 2009 H1N1 influenza July 2009 to June 2010 in a tertiary care hospital in Khamis Mushyt, in the southern region of Saudi Arabia and the hospital course of these patients outcome and the predictive factors associated with poor outcome.

Patients and Methods.
Study design: We performed a prospective study of all patients who were admitted to Internal Medicine or Pediatric Department, at Armed Forces Hospital Southern Region Khamis Mushyt From July 3, 2009, to June 1, 2010.
Selection of patients: We review all the records of hospitalized patients that had at least 48 hours with influenza-like illness (temperature of 37.8C or higher or cough or sore throat) and had 2009 H1N1 virus infection confirmed with RT-PCR at National Health Virology Laboratory in Riyadh. The RT-PCR assay was performed using kits of the Centers for Disease Control and Prevention (RT-PCR protocol for detection and characterization of swine influenza, version 2009, CDC REF.#I-007-05).[11]
We identified patients prospectively through daily reports regarding case-level information (including hospitalization status) from departmental reports and authors personally collected clinical information for each hospitalized patient after informed consent.
We gathered data from the hospitalized patients through standardized sheet record that included demographic data, influenza-vaccination history for the previous year, underlying medical conditions, clinical signs and symptoms, laboratory tests, radiographic findings, treatment course, requirement for ventilation or intensive care and eventual outcome. Specimens for bacterial infections were sent for all patients to rule out secondary infections. For time calculations, the day of admission was considered to be hospital day 0. The body-mass index (BMI, the weight in kilograms divided by the square of the height in meters) was calculated, for patients for whom height and weight were available, to determine whether the patient was obese (with obesity defined as a BMI of 29.9 or higher in adults 13 years of age or older or a BMI percentile of 95 to 100 in children between the ages of 2 and 12 years) or morbidly obese (BMI ≥39.9 in adults only); the BMI was calculated in pregnant women based on their first antenatal visit in medical charts. Fever was defined as documented temperature of ≥37.5C with high grade temperature being defined as documented temperature of ≥38.5C. Complicated pandemic influenza was defined in the presence of radiologically confirmed pneumonia, septic shock, multiorgan failure, central nervous system (CNS) involvement, etc. or secondary bacterial infections of the upper respiratory tract defined and diagnosed by standard methods. A severe case was defined as requiring admission to an intensive care unit (ICU) or death.  We performed separate analysis for patients who either died or were admitted to an ICU. We did a multivariate logistic-regression analysis to further investigate associations with the severity of illness.

Clinical features: A total of 52 patients (15 males) were adults and 65 were pediatrics (19 males). There were 15 patients in the age group 60 years and above, 35 patients in the age group 21-60 years, 18 patients in the age group 6-20 years and 41 patients in the age group 0-5 years (see table 1). The common presenting symptom associated with the disease was fever >38.5 C present in 85 cases (72.6%), dry cough 81 cases (69.2%), dyspnea 40 cases (34.5%), vomiting 40 cases (34.2%) and diarrhea 45 cases (38.8%). The most significant signs associated with pandemic 2009 H1N1 infection in our hospitalized patients were tachycardia present in 96 cases (83.5%), room air desaturation on pulse oximetry less than 95% present in 65 cases (55.6%), tachypnea more than 20 breaths per minute present in 82 cases (97.6%), and crackles on chest auscultation present in 57 cases (49.1%) (see table 1 for details).
Co-morbidities: There were 10 patients who were pregnant (10.5%) and the common co-morbidities among the all patients hospitalized with H1N1 pandemic were chronic kidney disease present in 5 cases (4.3 %), Sickle cell anemia present in 8 cases (6.9%), Congenital cardiovascular disease present in 5 cases (4.3%), Asthma present in 7 cases (6.8%) and Epilepsy present in 11 cases (9.5%).
Radiological and Laboratory Findings: Most of the patient hospitalized with 2009 H1N1 infection had right sided infiltrates on chest x-ray in 24 cases (20.5%) and normal chest x-ray in 28 patients (41.0%).
Arterial blood gases showed hypoxia in 47 patients (56.6%), while blood work showed anemia in 36 patients (30.8%), normal white blood count in 82 cases (70.1%), low white blood count in 11 patients (9.4%), raised alanine transaminase in 19 patients (16.8%)
Morbidity: The patients hospitalized with 2009 H1N1 infection had complications like requiring non-invasive ventilation in 4 patient (3.4%) and invasive mechanical ventilation in 16 cases (13.7%). The mortality in our patients was 18 cases (16.7%).
Pregnancy: A total of 10 patients who were pregnant (10.5 %), there were 0 deaths among pregnant patients and the mortality was slightly better when compared to the total population mortality of 16.7%.
Predictive Model: We tried to identify the factors predicting the poor outcome in the patient hospitalized with 2009 H1N1 infection. The important factors associated with morbidity and mortality were need for non-invasive mechanical ventilation (n=4), invasive mechanical ventilation (n=16), type 2 respiratory failure (n=7), bilateral chest infiltrates (n=11) and low white blood cell count (n=11) but the small number of patients did not allow to do more stratified analysis. The logistic regression analysis showed that the hypoxia at admission is the most important variable (n=65) after adjusted by duration of symptoms, age and sex, the model has an area under the curve being 0.768.
Predictive Model=Log P (dead)-3.03+1.85* Hypoxia +0.10 * Days of onset of symptoms +0.006 * Age+0.91*sex  (see figure 1).

Table 1.
Demographics and clinical features before and after admission in patient hospitalized with 2009 H1N1 infection.

Table 2.
Co-morbidities in patient hospitalized with 2009 H1N1 infection.

Table 3.
Pertinent investigations in patient hospitalized with 2009 H1N1 infection.

Table 4.
Complications in patient hospitalized with 2009 H1N1 infection.

Figure 1.
Predictive model for predicting poor outcome in patients hospitalized with 2009 h1n1 pandemic.

In this study, we have shown that pandemic influenza caused considerable morbidity in a significant proportion of hospitalized adults and that the use of antiviral drugs was beneficial in hospitalized patients. The distribution of influenza A (H1N1) cases by age was similar to the distribution of cases observed worldwide, suggesting the possibility of varying levels of immunity in the young age group although more than 90% of influenza-related deaths occur in patients in the older age group.[12] Underlying medical conditions that have been reported in patients worldwide who were hospitalized with seasonal influenza have included diabetes and cardiovascular, neurologic, and pulmonary diseases, including asthma.[13] Frequently reported complications have included pneumonia, bacterial coinfection, and exacerbation of underlying medical conditions, such as congestive heart failure.[14]
Our study summarizes the clinical findings regarding patients who were hospitalized for the treatment of 2009 H1N1 influenza in pandemic period over one year. Studies have shown that early therapy with oseltamivir in patients with 2009 H1N1 virus infection may reduce the duration of hospitalization and the risk of progression to severe disease requiring ICU admission or resulting in death.[15,16,17] Studies have also shown that patients with immunosuppressant states such as cancer and/or stem cell transplantation who develop 2009 H1N1 have not been associated with more poor outcome.[18] International studies have shown that In-hospital mortality was higher in patients with pneumonia than in the others (5.2% vs. 0%; p < 0.001) while the absence of comorbid conditions (odds ratio [OR], 2.07; 95% confidence interval [CI], 1.32-3.24) was found to be an independent risk factor for pneumonia, whereas early (≤48 h) oseltamivir therapy (OR, 0.29; 95% CI, 0.19-0.46) was a protective factor.[19]
When compared to the local studies in Saudi Arabia, risk factor associated with severe 2009 H1N1 infection leading to either complications or intensive care admission have been delay in initiation of antiviral therapy, history of opium inhalation, younger age group and in female dependents and certain symptoms like; productive cough, hemoptysis, chest pain, confusion, loss of consciousness.[20,21]
In our study involving 117 patients with 2009 H1N1 virus 40.2% had pneumonia on chest x-ray, 19.2% required mechanical ventilation and 16.7% died; all patients received oseltamivir. The factor most predictive of morbidity and mortality was hypoxia at admission that had an area under curve of 0.768 with OR for hypoxia being 6.356 with 95% CI being 1.16-34.55. Although the number of patients in this study was small and it was single centered experience, we may identify patient at high risk for morbidity and mortality using this model.

During the evaluation period, 2009 H1N1 influenza caused severe illness including pneumonia (40.2 %), intensive care unit admission (19.2%) and death (16.7%) in single tertiary care hospital in the southern region of Saudi Arabia over a one year period. The most significant factor in our small study that was predictive of morbidity and mortality in our patients was hypoxia at admission that had an area under curve of 0.768 (see figure 1). Identifying the patients with hypoxia in suspected cases of 2009 H1N1 influenza infection is therefore prudent as these patients may be at greater risk for morbidity and mortality. Although 2009 H1N1 infection is now considered to be in post-pandemic phase with low virological activity, identifying risk factors such as hypoxia on presentation in these patients may still be necessary to optimize treatment strategies and therefore further studies to needed identify/confirm the factors predicting poor outcomes in patients admitted with 2009 H1N1 infection.

The authors would like to thank Department of Preventive Medicine especially Dr. Noha, all doctors of Internal Medicine department and all the staff of Infectious Diseases team for their kind co-operation.


  1. Echevarra Zuno S, Meja-Arangur JM, Mar-Obeso AJ, et al. Infection and death from influenza A H1N1 virus in Mexico: a retrospective analysis. Lancet 2009; 374:2072-9. 
  2. Perez-Pailla R, de la Rosa-Zamboni D, Ponce de Leon S, et al. Pneumonia and respiratory failure from swine-origin influenza A (H1N1) in Mexico. N Engl J Med 2009; 361:680-9. PMid:19564631
  3. Novel Swine-Origin Influenza A (H1N1) Virus Investigation Team. Emergence of a novel swine-origin influenza A (H1N1) virus in humans. N Engl J Med 2009; 360: 2605-15. PMid:19423869
  4. Garten RJ, Davis CT, Russell CA, et al. Antigenic and genetic characteristics of swine-origin 2009 A (H1N1) influenza viruses circulating in humans. Science 2009; 325:197-201. PMid:19465683 PMCid:3250984
  5. Pandemic (H1N1) 2009 — update 94. Geneva: World Health Organization, April 1, 2010. 
  6. CDC estimates of 2009 H1N1 influenza cases, hospitalizations and deaths in the United States, April 2009–February 13, 2010. Atlanta: Centers for Disease Control and Prevention, 2010. 
  7. World Health Organization (WHO) Pandemic influenza vaccines: Current status. Available from: 
  8. Saudi Arabia confirms first H1N1 flu case. Available from: 
  9. Saudi Ministry of Health. The national plan for preventing Flu pandemics (1430 – 2009) Available from: 
  10. Alenzi FQ. H1N1 update review. Saudi Med J. 2010 Mar;31(3):235-46 PMid:20231926
  11. World Health Organization. CDC protocol of realtime RTPCR for swine influenza A (H1N1), version 2009. Available from: URL: 
  12. Thompson WW, Shay DK, Weintraub E, et al. Influenza-associated hospitalizations in the United States. JAMA 2004; 292: 1333-40. PMid:15367555
  13. Thompson WW, Shay DK, Weintraub E, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003; 289:179-86. 
  14. Schrag SJ, Shay DK, Gershman K, et al. Multistate surveillance for laboratory confirmed, influenza-associated hospitalizations in children: 2003-2004. Pediatr Infect Dis J 2006; 25:395-400. PMid:16645501
  15. Harper SA, Bradley JS, England JA, et al. Seasonal influenza in adults and children — diagnosis, treatment, chemoprophylaxis, and institutional outbreak management: clinical practice guidelines of the Infectious Diseases Society of America. Clin Infect Dis 2009; 48:1003-32. PMid:19281331
  16. WHO guidelines for pharmacological management of pandemic (H1N1) 2009 influenza and other influenza viruses. Geneva: World Health Organization, February 2010. 
  17. Patients hospitalized with 2009 pandemic influenza A (H1N1) — New York City, May 2009. MMWR Morb Mortal Wkly Rep 2010; 58:1436-40. PMid:20057350
  18. Redelman-Sidi G, Sepkowitz KA, Huang CK, et al. H1N1 influenza infection in cancer patients and hematopoietic stem cell transplant recipients. J Infect 2010; 60:257-63. PMid:20138188
  19. Viasus D, Pao-Pardo JR, Pachn J, Riera M, Lpez-Medrano F, Payeras A et al for the Novel Influenza A(H1N1) Study Group of the Spanish Network for Research in Infectious Diseases (REIPI). Pneumonia Complicating Pandemic (H1N1) 2009: Risk Factors, Clinical Features, and Outcomes. Medicine (Baltimore). 2011 Sep;90(5):328-336 
  20. Tabarsi P, Moradi A, Marjani M, Baghaei P, Hashemian SM, Nadji SA, Fakharian A, Mansouri D, Masjedi M, Velayati A. Factors associated with death or intensive care unit admission due to pandemic 2009 influenza A (H1N1) infection. Ann Thorac Med. 2011 Apr;6(2):91-5. PMid:21572699 PMCid:3081563
  21. Herzallah HK, Bubshait SA, Antony AK, Al-Otaibi ST. Incidence of influenza A H1N1 2009 infection in Eastern Saudi Arabian hospitals. Saudi Med J. 2011 Jun;32(6):598-602. PMid:21666942

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