ANTIFUNGAL SUSCEPTIBILITY TESTING: CURRENT ROLE FROM THE CLINICAL LABORATORY PERSPECTIVE

Brunella Posteraro, Riccardo Torelli, Elena De Carolis, Patrizia Posteraro, Maurizio Sanguinetti
  • Brunella Posteraro
    Catholic University. Public Health, Italy
  • Riccardo Torelli
    Catholic University. Microbiology, Italy
  • Elena De Carolis
    Catholic University. Microbiology, Italy
  • Patrizia Posteraro
    Clinical Laboratory, Ospedale San Carlo, Rome, Italy

Abstract

Despite availability of many antifungal agents, antifungal clinical resistance occurs, perhaps as a result of an infecting organism found to be resistant in vitro to one or more antifungals tested. Thus, antifungal susceptibility testing (AFST) results, if timely generated by the clinical microbiology and communicated to clinicians, can aid them in the therapeutic decision making, especially for difficult-to-treat invasive candidiasis and aspergillosis. Although recently refined AFST methods are commercially available to allow a close antifungal resistance surveillance in many clinical setting, novel assays, relying on short-time antifungal drug exposure of fungal isolates, are upcoming tools for AFST. Based on emerging technologies such as flow cytometry, MALDI-TOF mass spectrometry, and isothermal microcalorimetry, these assays could provide a reliable means for quicker and sensitive assessment of AFST.

Keywords

Fungal infections, Antifungal agents; Susceptibility testing

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Submitted: 2014-07-21 14:06:01
Published: 2014-04-07 00:00:00
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