Prevalence of Alpha Thalassemia in Microcytic Anemia: a Tertiary Care Experience from North India
Monica Sharma, Sanjay Pandey, Ravi Ranjan, Tulika Seth and Renu Saxena
Department of Hematology, AIIMS, New Delhi
Received: August 20, 2014
Accepted: November 24, 2014
Mediterr J Hematol Infect Dis 2015, 7(1): e2015004, DOI 10.4084/MJHID.2015.004
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AbstractIntroduction. Cases with microcytosis not responding adequately to iron supplementation are diagnostic dilemma and have been reported to harbor alpha (α) thalassemia mutations. The aim of this study was to determine the common α globin gene deletions in cases with microcytic anemia.
Methods Fifty four patients selected (22 females and 32 males) had microcytic anemia (MCV < 80 fl, Hb <12gm/dl) with raised TRBC (> 5M/mm3) but normal Hb HPLC. They had either low or normal Transferrin Saturation (TS). Gap-PCR for four common α-gene deletions (-α3.7, -α4.2, - -αSA and --αSEA) was done.
Results Out of the total fifty-four cases nineteen (35.2%) cases were found to have α gene mutations; Three homozygous and sixteen heterozygous cases including -α3.7 deletions and a single case of --αSA ; but no -α4.2 and –SEA mutations were found.
Conclusion α gene mutations can confound iron deficiency anemia, but no RBC indices, or a discriminant function can identify it is presence Molecular studies have to be resorted to. Gap PCR for common α thalassemia mutation including –αSA should be done even in the face of low iron stores in subjects who respond incompletely to iron supplementation.
Iron deficiency anemia (IDA), β thalassemia trait (βTT) and anemia
of chronic disease (ACD) are common causes of microcytosis that can be
diagnosed accurately by Iron studies and Hb HPLC respectively. However,
when HbA2 level is normal or low along with normal or low serum iron
studies, microcytosis can be a diagnostic dilemma. Walford et al. and
Pearson et al.[1,2] had long back suggested that such cases could be
harboring α thalassemia mutation.
The α -thalassemia syndromes are among the most common single-gene disorders with more than 20% of the world population to be a carrier of some form of α –thalassemia, as estimated by The World Health Organization (WHO).[3-5] In India also it is common, and the gene frequency of occurrence is appreciably higher than that of βTT. Deletion - α3.7 is the commonest reported genotype which exists in α + (-α/αα) milder form.[7,8] Most alpha thalassemia cases in India have been reported among tribal populations[8,9] with βTT and other hemoglobinopathies but its co-existence with IDA has never been studied.[7,10-13] Iron deficiency is highly prevalent in India and with the reportedly high frequency of α thalassemia the likelihood of the two conditions coexisting can be expected to be high. The Discrimination between microcytosis due to the two conditions is not only clinically significant but is often difficult even more so when they coexist. No Discriminant function or RBC Indices can indicate the presence of α thalassemia in the subjects with or without IDA.[14-16] Molecular studies most commonly deletion-specific gap-PCR have to be resorted to for detection of the common α0- and α-thalassemia deletions in such cases when the Hemoglobin or microcytosis does not improve appropriately after iron supplementation.
The mutation spectrum for each population group is distinct characterized by a small number of founder mutations that reflect the predominant mutant alleles in carriers and affected individuals.[17,18] This helps in designing the gap PCR assay individually or in multiplex panels. One of the most popular multiplex assays covers seven deletions, specifically the SEA, FIL, MED-I, THAI, 20.5, 3.7, and 4.2 deletions.[19-22] Other molecular studies though not readily available are multiplex ligation-dependent probe amplification (MLPA) /microarray analysis, allele-specific assays and α -globin gene resequencing for detection of common, rare, and private point mutations.
Based on previous studies in India in our laboratory, gap PCR for - α3.7, -α4.2,- - αSA and - - αSEA deletions are done.
Microcytic hypochromic anemia is the hallmark feature of α thalassemia, and the degree of microcytosis is directly proportional to the number of alpha genes deleted, thus, reflecting the rate of imbalance between α - and β-chain expression.[23,24] Apart from few studies,[15,25] there is a paucity of population-based studies on MCV in α thalassemia observing the effect of its variation in alpha genes deletions & also it is an alteration with the coexistent IDA.
This study was undertaken to identify and highlight the presence of common α thalassemia deletions in cases of microcytic anemia and its interaction with IDA.
Materials and methods
Fifty-four Patients, attending Hematology outpatient department, All
India Institute of Medical Sciences, New Delhi were included in the
study that was approved by institutional ethical committee. Duration of
the study was 1.5 years between 2010 and 2011.The cases included had
Hemoglobin <12 gm/dl, MCV<80fl and TRBC > 4.5 M/mm3. Only
cases with standard Hb HPLC showing low or normal HbA2 (< 3.5 %)
were taken to exclude βTT/Hemoglobinopathy.
Sampling: 3 ml blood in EDTA for CBC and Hb HPLC and 1 ml blood in 3.2% sodium citrate for DNA studies was obtained after taking signed consent from the patients.
Hematological work up: Hematological parameters were analyzed by automated cell counter (XT1800i, Sysmex Transasia). Quantification of HbA2 was performed by High Performance Liquid Chromatography (Hb-HPLC, Bio-Rad variant- β thalassemia short program). Serum Iron studies were performed by standard laboratory methods and transferrin saturation (TS) <16% was taken to be iron-deficient.
Detection of the α thalassemia mutations: Genomic DNA was prepared from peripheral blood by the standard phenol-chloroform extraction method. Deletion mutations were characterized by Gap-PCR. Detection for single deletion - α3.7 and -α4.2 Baysal et al. - - αSA deletion Shahji et al., and - - αSEA Chang et al. was done.
Follow-up: Though it was intended to be a cross-sectional study, the Follow-up data on Iron therapy available were evaluated in 27 (54%) cases.
Statistical analysis: The following parameters were assessed: Hemoglobin, MCV, TRBC, RDW-CV, TS and HbA2. Mutation positive and negative groups were compared. Statistical analysis was carried out using the statistical package SPSS version, and an independent sample t-test was used for comparison of hematological parameters. Statistical significance was assessed as a test with p ≤ 0.05.The hematological parameters of three alpha thalassemia mutations, the four groups based on mutation status and TS < / > 16 % were also compared, but the size in each group was too small for obtaining a valid statistical comparison.
Of the 54 cases included in the study, 32 (57.4%) were males and 22
(42.6%) females and the median age was 19.5 years (13 months - 62
years). There were four pediatric cases (<18 years) in alpha
positive genotype group and seven in wild alpha genotype group. The
subjects were, for the most, anemic (median Hemoglobin was 9.6 g/dl
(5.1-12g/dl), with RBC showing microcytosis (median MCV63.4fl (48-79
fl) and iron deficiency (median TS 10.2% (7.6-19.2%).They had TRBC
median 5.2M/mm3 (4.5-6.2M/mm3), median RDWCV 19.3 %( 14-29%) and median HbA2 2.3 %( 1.6-2.8%).
Characteristics of alpha thalassemia subjects: Nineteen out of 54 (35.2 %) cases showed α thalassemia deletions and three genotypes were seen on gel electrophoresis. Fifteen were –α3.7 heterozygous, three were α3.7 homozygous and one was - - αSA double deletion (Figure 1 and 2). Comparative hematological parameters of mutation-positive and mutation-negative cases are given in table 1. The subjects had presented chiefly for work up for long standing anemia or detected incidentally and few for nonimmune hydrops. The median hemoglobin was 10.5 g/dl (5.9-11.9g/dl) across the α thalassemia genotypes. The median MCV was significantly lower in the three homozygous with α3.7 deletions, median 56.2fl (56-72.4fl), compared to the median, 68.9fl (47.6-80fl) (29.6%), of the subjects -a3.7 deletion heterozygous and to the value, 71.6fl, of the one subject (1.9%) –with αSA thalassemia.
Figure 1. α-3.7 kb mutations. (Lane 1A, 2A,3A, 4A, and 5A are normal set and lane 1B, 2B, 3B, 4B and 5B are mutant set). Lane 1AB patient homozygous, 2AB normal, 3AB heterozygous, 4AB normal and 5AB Heterozygous. Primer A+C (A) normal and A+ B(B) mutant are amplified in separate tubes because their product have same size of1.8 kb - Baysal et al.
|Figure 2. α—SA Mutations. (Lane 1A, 2A,3A are mutant set and lane 1B, 2B, 3B are normal set). Lane 1AB heterozygous, 2AB homozygous and 3AB is normal. Amplification done according to Shahji et al.|
There were high RDW-CV and low %TS indicating coexisting IDA
in 10 of the 19 mutation-positive cases. On comparing the hematological
parameters of α thalassemia mutation-positive and negative cases (Table 1).
The mean Hemoglobin and MCV were better in the mutation-positive cases
while the mean RDW-CV was higher in the mutation-negative group
(Hemoglobin, MCV p=0.161, p=0.151, RDW-CV and TS p=0.040, 0.001
respectively). Interestingly 28/35(80%) mutation negative cases showed
raised TRBC (Table 2) suggesting that a high >5M/mm3 TRBC may not always be thalassemia.
Effect of coexisting iron deficiency on α thalassemia RBC indices (Table 3): The median Hemoglobin, MCV, and HbA2 were lower in both the iron deficient groups 1 and three, but the values were relatively much lower in the mutation-negative group 1which was predominantly IDA. TRBC was high in both the groups. Between groups 2 & 4 which were both iron sufficient (i.e. TS > 16%) the median Hemoglobin, MCV, TRBC, and HbA2 were lower in group 4 (mutation positive) compared to group2. The RDW-CV was comparable indicating the coexisting IDA in both the groups. This shows that MCV is lower in α thalassemia compared to Normal. Follow up on Iron was available in 27 (54.00%) cases. Response was seen predominantly (63.1%) in group 1 as expected but 36.8% in this group also failed to respond no details about the compliance or cause of refractoriness was available. Subjects of Group 2 did not require iron, but they needed to be investigated for other underlying Hemoglobinopathy, which was beyond the scope of this study. In mutation-positive group 3 (50%) patients showed no or inadequate response to iron therapy while individuals in group 4 had % TS >16% and did not need iron supplementation. The family history too was significant only in individuals of the group 4.
|Table 1. The baseline hematological profile & TS% of mutation positive & negative cases (n=54)|
|Table 2. TS% and TRBC between mutation positive and negative case|
|Table 3. Effect of iron deficiency on Hematological parameters with and without α thalassemia mutation|
Fifty-four subjects with microcytosis but with normal Hb HPLC and
normal or low Iron studies were evaluated for the presence of common α
-thalassemia deletion mutations. Microcytosis was the defining criteria
in the study, but it is also important to remember that normal RBC
indices do not rule out α thalassemia carrier. In the current study
with gap, PCR α thalassemia could be detected in nearly 1/3rd
(35.2% -19/54 cases) of the microcytic cases. These results agree with
50% a-thalassemia cases reported in non-anemic microcytosis cases by
Borges et al. in southeastern Brazilian population and the other
studies in European or European-derived populations who also have
reported a-thalassemia trait in 25%-80% of non-anemic subjects with
microcytosis without iron deficiency.[30,31] As reported in various
Indian studies -α3.7 deletion was the
commonest determinant in the study seen predominantly in the
heterozygous state (31.7% of microcytic patients).[7,11] Interestingly
a single case of --αSA was detected incidentally. Though 5% and 3.33% cases of -α4.2 , --αSEA
deletion respectively have been reported in Indian subjects by Sarkar
et al. but none was seen in the study which could have been due to
the difference in the ethnic group which in this study was largely
North Indian. This also could explain the high frequency of
a-thalassemia cases observed even when only microcytic subjects were
investigated, and uncommon α thalassemia deletions and point mutations
could not be done. This is concurrent with the observations of K Ghosh
et al who have reported highest prevalence in the Punjabis.
Phenotypically all the 19 α thalassemia cases were very similar presenting with only mild anemia. None of the cases had jaundice or gall stone disease. Family history was positive in four individuals. Microcytosis was the defining criteria and was most pronounced in the three (5.6%) -α3.7 deletion homozygous individuals compared to (29.6%) -α3.7 deletion heterozygous and one (1.9%) --αSA thalassemia subjects. Though insufficient in number, the findings are largely in concordance with previous reports, where microcytosis has been explained on the basis of α-gene number.[29,32-36] IDA causes the Indices to be much lower than in α thalassemia and may have high TRBC in pediatric group and after recent iron supplementation.[1,2,37,38] A report on alpha thalassemia with anemia in children’s revealed it should be considered differential diagnosis. On comparing the hematological parameters within the four groups, group 1( IDA cases) had the most pronounced microcytosis even more than the cases with IDA coexistent with α thalassemia mutation (group 3) which had more than that of α thalassemia cases (group 4). (MCV group1 <group3< group4 < group2). The subjects of Group 3 also had much lower RBC indices than that of group 4 which was akin to normal individuals. In conclusion, Using low Hemoglobin & MCV ,raised TRBC, normal Hb A2 and deletion specific gap- PCR for common alpha thalassemia mutations (including - -αSA), nearly 1/3 (35.2%) of the 54 cases of microcytic anemia with or without IDA could be typed in the study. Finding of IDA should not be a deterrent for the screening of a coexisting α thalassemia mutation, which is particularly relevant in a country like India where both α thalassemia (in some geographical areas) and IDA occur with high frequency. Larger population studies, including other less common α thalassemia mutations, are required not only to identify the population group specific mutation spectrum but also to observe the interplaying of the two conditions on the RBC indices. In fact red cell indices could differentiate or indicate the coexistence of the two conditions. Until then molecular studies (gap-PCR) should be done for the detection of common α thalassemia mutations especially when the Hemoglobin or microcytosis does not improve appropriately on iron supplementation this can be useful in the countries where α thalassemia is not prevalent because as a consequence of population migrations alpha thalassemia has acquired a truly global distribution.
- Walford DM, Deacon R. A thalassemia trait in various racial groups in the United Kingdom: characterization of alpha thalassemia in Indians. Br J Haematol. 1976; 34:193-206. http://dx.doi.org/10.1111/j.1365-2141.1976.tb00190.x
- Pearson, H. A., McPhedran, P, O'Brien, R. T., Aspnes, G. T., McIntosh, S., and Guiliotis, D. K.Comprehensive testing for thalassemia trait. Annals of the New York Academy of Sciences.1974; 232:135-144. http://dx.doi.org/10.1111/j.1749-6632.1974.tb20578.x PMid:4528485
- Modell B, Darlison M. Global epidemiology of hemoglobin disorders and derived service indicators. Bull WHO 2008; 86:480-7. http://dx.doi.org/10.2471/BLT.06.036673 PMid:18568278 PMCid:PMC2647473
- Weatherall DJ, Clegg TB. The thalassemia syndromes, 4th ed. Oxford: Blackwell Science; 2001: 267p. http://dx.doi.org/10.1002/9780470696705
- Shaji RV, Eunice SE, Baidya S, Srivastava A, Chandy M.Determination of the breakpoint and molecular diagnosis of a common a-thalassemia deletion in the Indian population. Br J Haematol. 2003; 123:942-47. http://dx.doi.org/10.1046/j.1365-141.2003.04704.x PMid:14632787
- Agarwal S, Pradhan M, Gupta U. R., Samai S, Agarwal S. S. Geo-graphic & ethnic distribution of ß-Thalassemia Mutations in Uttar Pradesh, India. Hemoglobin. 2000; 24: 89-97. http://dx.doi.org/10.3109/03630260009003427 PMid:10870879
- Nadkarni A, Phanasgaonkar S, Colah R, Mohanty D, Ghosh K. Prevalence and molecular characterization of alpha-thalassemia syndromes among Indians. Genet Test.2008; 12:177-80 http://dx.doi.org/10.1089/gte.2007.0080 PMid:18447636
- Balgir R. S.The burden of haemoglobinopathies in India and the challenges ahead. Current Science. 2000;79:1536-1547
- Dastidar R and Talukder G. The Molecular Basis of Alpha-Thalassaemias in India: A Review Anthropologist Special. 2007; 3: 349-354.
- Panigrahi I, Rafeeq PHA, Choudhry VP, Saxena R. High frequency of deletional a thalassemia in ß thalassemia trait: Implications for genetic counseling. Am J Hematol. 2004; 76:297-29. http://dx.doi.org/10.1002/ajh.20083 PMid:15224373
- Sankar VH, Arya V, Tewari D, Gupta UR, Pradhan M, Agarwal S. Genotyping of alpha-thalassemia in microcytic hypochromic anemia patients from North India. J Appl Genet. 2006; 47(4):391-5. http://dx.doi.org/10.1007/BF03194650 PMid:17132905
- Bandopadhyay S, Roychowdhury K, Chandra S Das M, Dasgupta UB. Variable severity of ß thalassemia patients of eastern India: effect of a thalassemia and Xmn1 polymorhism.Clin Exp Med.2001;1:155-159. http://dx.doi.org/10.1007/s10238-001-8028-x
- Kulozik AE, Kar BC, Serjeant GR, Serjeant BE, Weatherall OJ. The molecular basis of a thalassaemia in India. Its interaction with the sickle cell gene. Blood. 1998; 71(2):467-72.
- England JM., Bain BJ, Fraser PM. Differentiation of iron deficiency from thalassaemia trait by routine blood-count. Lancet. 1973; 1: 449-452. http://dx.doi.org/10.1016/S0140-6736(73)91878-3
- Souid AK, Nagelkerke N, Showqi S, Balhaj G. Erythrocyte reference values in Emirati people with and without alpha thalassemia. BMC Blood Disord. 2011; 11:1. http://dx.doi.org/10.1186/1471-2326-11-1 PMid:21345240 PMCid:PMC3053240
- Hegde UM, White JM, Hart GH, Marsh GM. Diagnosis of alpha-thalassaemia trait from Coulter Counter 'S' indices.J clin. Path. 1977;30:884-889
- Bernini LF. Geographic distribution of a thalassemia. In: Disorders of Hemoglobin: Genetics, Pathophysiology, and Clinical Management, 1st edn. Steinberg MA, Forget BG, Higgs DR, Nagel RL (eds). Cambridge, UK: Cambridge University Press, 2001 878-94.
- Harteveld CL, Higgs DR. a-Thalassaemia. Orphanet J Rare Dis 2010; 5:13. http://dx.doi.org/10.1186/1750-1172-5-13 PMid:20507641 PMCid:PMC2887799
- Chong SS, Boehm CD, Cutting GR, Higgs DR. Simplified multiplex-PCR diagnosis of a-thalassemia. Clin Chem 2000; 46:1692-5. PMid:11017952
- Chong SS, Boehm CD, Higgs DR, Cutting GR. Single-tube multiplex-PCR screen for common deletional determinants of a-thalassemia. Blood 2000; 95:360-2. PMid:10607725
- Tan AS, Quah TC, Low PS, Chong SS. A rapid and reliable 7-deletion multiplex polymerase chain reaction assay for a-thalassemia. Blood 2001; 98:250-1. http://dx.doi.org/10.1182/blood.V98.1.250 PMid:11439976
- Kidd JK, Azimi M, Lubin B, Vichinsky E, Hoppe C. Application of an expanded multiplex genotyping assay for the simultaneous detection of hemoglobin Constant Spring and common deletional a-thalassemia mutations. Int J Lab Hematol 2010; 32:373-80. PMid:19919622
- Higgs DR, Buckle V, Gibbons R, Steensma D. Alpha-Thalassaemia. Baillieres. Clin Haematol 1993, 6:117-150. http://dx.doi.org/10.1016/S0950-3536(05)80068-X
- Bain BJ: Hemoglobinopathy diagnosis second edition. Blackwell Publishing Ltd; 2006. http://dx.doi.org/10.1002/9780470988787
- Beutlar E, West C. Hematologic differences between African-Americans and whites: the roles of iron deficiency and a thalassemia on hemoglobin levels and mean corpuscular volume. Blood. 2005; 106(2):740-745. http://dx.doi.org/10.1182/blood-2005-02-0713 PMid:15790781 PMCid:PMC1895180
- Baysal E, Huisman TH. Detection of common deletional alpha thalassemia 2 determinants by PCR. Am J Hematol.1994; 46:208-13. http://dx.doi.org/10.1002/ajh.2830460309
- Chang JG, Lee LS, Lin CP, Chen PH. Rapid diagnosis of alpha thalassemia-1 of Southeast Asia type and hydrops fetalis by polymerase chain reaction. Blood. 1991; 78:853-4. PMid:1859898
- Higgs DR, Vickers MA, Wilkie AO, Pretorius IM, Jarman AP, Weatherall DJ.A review of the molecular genetics of the human a globin gene cluster. Blood. 1989; 73:1081-104 PMid:2649166
- Borges E, Wenning MR, Kimura EM. High prevalence of a thalassemia among individuals with microcytosis and hypochromia without anemia. Braz J Med Biol Res. 2001; 34:759-62. http://dx.doi.org/10.1590/S0100-879X2001000600009 PMid:11378664
- Borges E, Wenning MR, Kimura EM, Gervasio SA, Costa FF, Sonati MF. Prevalence of alpha-globin gene deletions among patients with unexplained microcytosis in a north-American population. Hemoglobin.2005; 29:51-60. http://dx.doi.org/10.1081/HEM-47024
- Sivera P, Roetto A, Mazza U, Camaschella C. Feasibility of molecular diagnosis of a thalassemia in the evaluation of microcytosis. Haematologica.1997; 82:592-3. PMid:9407728
- Sarkar AA, Banerjee S, Chandra S, Ghosh M. A novel 33.3 kb deletion (- - KOL) in the alpha globin gene cluster: a brief report on deletional alpha thalassemia in the heterogenous eastern Indian population.Br J Haematology.2005; 130: 454-457. http://dx.doi.org/10.1111/j.1365-2141.2005.05626.x PMid:16042697
- Ko TM, Hwa HL, Liu CW Li SF, Chu JY, Cheung YP. Prevalence study and molecular characterization of alpha thalassemia in Filipinos. Annals of Hematology.1999; 78: 355-357. http://dx.doi.org/10.1007/s002770050528
- Mach-Pascual S, Darbelly R, Pilotto PA, Beris P. Investigation of microcytosis: A comprehensive approach. Eur J Haematol.1997; 57:54-61. http://dx.doi.org/10.1111/j.1600-0609.1996.tb00490.x
- Wagner SC, de CastroSM, Tatiana P, Gonzalez, Ana P. Santin, Leticia Filippon, Prevalence of common alpha thalassemia determinants in south Brazil: Importance for the diagnosis of microcytic anemia. Genetics and Molecular Biology.2010; 33(4): 641-645. http://dx.doi.org/10.1590/S1415-47572010005000086 PMid:21637571 PMCid:PMC3036136
- Garshasbi M, Oberkanins C, Law HY Neishabury M Kariminejad R, Najmabadi H. Alpha-globin gene deletion and point mutation analysis among Iranian patients with microcytic hypochromic anemia.Haematologica.2003;88(10):1196-7. PMid:14555321
- Pornpatkul M, Wasi P, Na-Nakorn S. Hematologic parameters in obligatory alpha thalassaemia traits. Journal of the Medical Association of Thailand.1969; 52:801-809.
- Aslan D, Altay C. Incidence of high erythrocyte count in infants and young children with iron deficiency anemia: re-evaluation of an old parameter. J Pediatr Hematol Oncol.2003; 25(4):303-6. http://dx.doi.org/10.1097/00043426-200304000-00007
Borato Viana,Benigna Maria de Oliveira. Alpha-thalassemia should be
considered in the differential diagnosis.of a child with anemia. Jornal
de Pediatria.2011;87(2) :180-82 http://dx.doi.org/10.1590/S0021-75572011000200016
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