Monticelli

Brain Abscesses Caused by Nocardia paucivorans in a Multiple Myeloma Patient Treated with Lenalidomide and Dexamethasone: a Case Report and Review of Literature

Jacopo Monticelli1, Roberto Luzzati1, Cristina Maurel1, Chiara Rosin1, Romina Valentinotti1 and Claudio Farina2

1 Unit of Infectious Diseases, University Hospital, Trieste, Italy.
2 Microbiology and Virology Institute, AO "Papa Giovanni XXIII", Bergamo, Italy. 

Corresponding author: Jacopo Monticelli. Unit of Infectious Diseases, University Hospital, Trieste, Italy.; Tel. (Unit of Infectious Diseases): +39-040-3992594; Fax: +39-0403992652. E-mail: jacopo.mont@hotmail.it

Published: January 1, 2015
Received: October 16, 2014
Accepted: December 18, 2014
Mediterr J Hematol Infect Dis 2015, 7(1): e2015011, DOI 10.4084/MJHID.2015.011
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Abstract

We report the first case of multiple brain abscesses caused by Nocardia paucivorans in a patient suffering from multiple myeloma on treatment with lenalidomide and dexamethasone. N. paucivorans is a recently described species of the genus Nocardia, which is supposed to have a heightened neurotropism in cases of disseminated infection. Although nocardiosis itself is an uncommon infectious complication in multiple myeloma so far, nocardial brain abscess should be added to the spectrum of adverse effects due to this novel chemotherapy regimen. 

Case Presentation

A 70-year-old male was transferred to our unit from the intensive care unit of a nearby hospital. He had had  sudden onset  clonic seizures involving the right hemisoma and the assessment of multiple brain abscesses by computerized tomography (CT) scan. The patient was receiving empirical treatment with metronidazole and cefotaxime. His past medical history included a 2-year-history of IgA myeloma (International Staging System – II). The patient was initially treated with melphalan plus prednisone plus bortezomib (4 cycles) according to MPV protocol. Then, he started treatment with lenalidomide and dexamethasone having completed eleven cycles of such regimen with partial response. During the chemotherapy regimen the patient did not receive a trimethoprim-sulfamethoxazole (TMP-SMX) prophylaxis and, at time of admission, we discontinued the chemotherapy regimen for myeloma. The patient suffered from a 2-month history of subcutaneous abscess of unknown origin on the left shoulder. The lesion was drained, and  the microbiological analysis was not conclusive because of mixed bacterial flora. The patient was living in a small rural hamlet. He was a construction worker, but he retired 10 years before the present hospital admission.
At hospital presentation, body temperature was 36.5 °C, heart rate was 92/min, blood pressure was 150/90 mmHg and Glasgow Coma Scale was 15/15. The patient was alert, oriented and no abnormal physical findings were shown, except for a painful hypoesthesia in the lateral and anterior sides of the left thigh, in the lateral side of the left leg and, on the dorsal side of the left foot. The subcutaneous abscess on the left shoulder  completely resolved  with no signs of local inflammation. Laboratory showed the following abnormalities: serum C-reactive protein 96.19 nmol/L (normal values inferior to 71.43 nmol/L), hemoglobin 90 g/L, white blood cells 3.9 x 109/L (51% neutrophil granulocytes). In addition, the analysis of peripheral blood lymphocytes subsets showed CD3+ 742 cells/µL, CD3+CD4+ 420 cells/µL (47%), CD3+CD8+ 241 cells/µL (27%), CD4+/CD8+ ratio 1.70, CD19+ 72 cells/µL. Serum protein electrophoresis showed a relative hypoalbuminemia (55.4%) according to the known monoclonal gammopathy (serum IgA 4490 mg/L, monoclonal component 3 g/L). The chest X-ray revealed a lower right lobe infiltrate associated with bilateral pleural effusion. A brain CT scan (Figure 1) with contrast medium showed 6 focal ring-enhancing lesions of which the largest one had a maximum diameter of 16 mm (left occipital lobe). All the lesions had a minimal perilesional oedema. The right frontal lesions led to a mass effect on the right lateral ventricle. At that time, the patient underwent CT-guided brain biopsy of a parietal lesion. A bioptic sample revealed branching Gram positive nocardioform rods. Then, cultures were incubated aerobically at 35°C for three weeks. All whitish, chalky and wet hay-smelling colonies (Figure 2) yielded on blood agar were stained by partial acid-fast method according to Kinyoun technique modified for nocardioforms (Figure 3).

Figure 1 Figure 1. Axial brain CT scan post-contrast medium administration. Images obtained before therapy show two ring-enhancing lesions with abundant peripheral oedema in right parietal lobe (a) and one in left parietal lobe at the vertex (b). Images obtained after therapy (c,d) show the complete resolution of the lesions.

Figure 2
Figure 2. Whitish chalky colonies of N. paucivorans yielded on blood agar.

Figure 3
Figure 3. N. paucivorans hyphae. Staining by partial acid-fast method according to Kinyoun technique modified for nocardioforms. The vegetative hyphae are well developed with irregular branches.

The suspicion that this strain could belong to Actinomycetales genus was based on its failure to clear casein (Biorad, France), on the resistance to lysozime and 5-fluorouracil, and on the enzymatic profile, by the API ZYM system (bioMérieux, France).[1-2] Identification was completed by the evaluation of the absence of urease after growth on Christensen urea agar and incubation at 25°C for three weeks and of the incapacity to clear casein (Biorad, France).  Finally, genotypical identification has been performed by amplification and sequencing of a DNA fragment coding a rRNA 16S according with the manufacturer’s (Applied Biosystem, USA) suggestions. Sequences have been compared with the reference ones present in the Gen Bank (NCB) database by the BLAST program. The sequence has been deposited in the GenBank (accession number: AY262324.1). Sequences producing significant alignments permitted to identify the strains as N. paucivorans with 100% value of identity with the reference strain.  
Minimal Inhibitory Concentrations' (MIC) testing was performed by the Epsilometer (E test, bioMérieux, France) testing. MICs' results were the following: amikacin 0.064 µg/mL, amoxicillin plus clavulanic acid (>256 µg/mL), ceftriaxone (0.75 µg/mL), cefepime (6 µg/mL), ciprofloxacin (0.047 µg/mL), clarithromycin (1 µg/mL), gentamicin (8 µg/mL), imipenem (0.19 µg/mL), linezolid (0.125 µg/mL), tobramycin (0.047 µg/mL), trimethoprim plus sulfonamide (0.002 µg/mL). Susceptibility testing was performed on Mueller Hinton Agar (bioMérieux). The results were read after 24 h of incubation at 37°C. Susceptibility testing was revised and interpreted according to CLSI criteria.[3] In order to exclude concomitant pulmonary nocardiosis, a diagnostic bronchoscopy was done but the microbiological analysis of the bronchoalveolar lavage isolated only commensal microbial flora. As a matter of fact, based on the initial identification and the preliminary antimicrobial susceptibility testing, intravenous TMP-SMX plus meropenem were administered for 24 days. Such antibiotic regimen was switched to oral ciprofloxacin for an overall treatment period of 12 months. The patient is doing well, his painful hypoesthesias have disappeared; no more seizure episodes and adverse effects due to long term antibiotic therapy occurred. The brain CT-scan monitoring showed a progressive reduction of all cerebral lesions (Figure 1). 

Discussion

Nocardia (order Actinomycetales) is a complex genus of Gram-positive and partially acid- and alcohol-fast bacteria forming irregular branching colonies on agar, including Nocardia cyriacigeorgica, Nocardia farcinica, Nocardia brasiliensis and Nocardia otitidiscaviarum, and other Nocardia species. N. paucivorans is a recently described species, identified by biochemical characteristics and 16S rDNA sequence analysis in bronchial secretions of a patient with chronic lung disease.[4,5]
Human nocardiosis is usually recognized as a sporadic, community-acquired infection.[6] Human nocardiosis affects both immunocompromised and immunocompetent hosts. Within the immunocompromising conditions, nocardiosis seems to affect primarily intravenous drug abusers, patients on systemic corticosteroids, AIDS patients, recipients of solid organ and bone marrow transplants, patients affected by chronic granulomatous diseases and hematologic malignancies. Host resistance to nocardial infection depends on cell-mediated immunity. Infection mainly occurs through direct skin inoculation or inhalation, and possible clinical manifestations are pulmonary nocardiosis, cutaneous and subcutaneous nocardiosis and systemic nocardiosis, including central nervous system (CNS) dissemination.[7-8] CNS nocardiosis can be the result of prior pulmonary infection or can exist on its own. A recent literature review on the nocardiosis reported that CNS involvement can manifest mainly as cerebral abscesses but also as meningitis or both brain abscess and meningitis.[9] The major species reported among the patients in this literature review were Nocardia asteroides (35%), N. farcinica (19%), and N. cyriacigeorgica (6%), even if Nocardia nomenclature dramatically changed in the last years complicating all etiological consideration.  Other less common species included Nocardia transvalensis (4%), N. brasilensis (3.6%), and N. otitidiscaviarum (2%) and others. Our patient had CNS nocardiosis due to N. paucivorans. The largest series of N. paucivorans infections published to date included 33 cases collected in northeast Australia over a 20-years span, with dissemination in at least 33% of cases and CNS infection in 80% of disseminated cases. Therefore, it was hypothesized that N. paucivorans had an enhanced neurotropism.[10-11]
Nocardial infection in multiple myeloma is quite a sporadic finding, often linked as a complication of bone marrow transplants.[12] There is limited literature on nocardiosis in multiple myeloma patients that did not undergo bone marrow transplant, and there are still fewer cases of nocardial infections that occurred during novel anti-myeloma treatments. For instance, it was recently reported cases of brain abscesses by N. cyriacigeorgica in two patients treated with bortezomib.[13] A PubMed search, crossing “Nocardia paucivorans” and “myeloma”, revealed no cases, and the N. paucivorans infections published so far in English literature did not occur in multiple myeloma patients.[10-11,13-15] Therefore, to the best of our knowledge, the present case is the first infection due to N. paucivorans in a patient affected by multiple myeloma.
Our patient had multiple risk factors for nocardiosis. The myeloma-related immunodeficiency includes mainly B cell dysfunction and cellular immunodeficiency[12,16] with significantly reduced numbers of NK and T cells and impaired T cells function.[17] Lenalidomide is a synthetic derivative of thalidomide, used in multiple myeloma for its immunomodulatory properties. The molecule induces a IL-2-mediated primary T cell proliferation with a concomitant increase in IFN-γ production along with anti-angiogenic, anti-proliferative, and pro-apoptotic properties.[18] However, lenalidomide combination therapy has shown to be prone to an increased risk of infections. In fact, a meta-analysis of efficacy and safety of lenalidomide for multiple myeloma showed an increased risk of infections comparing lenalidomide regimen versus placebo (RR = 1.98; 95% CI: 1.50 to 2.62).[19] Therefore, an antibiotic prophylaxis should be considered as part of the treatment regimen.[19] Bortezomib induces apoptosis preferentially in rapidly proliferating and neoplastic cells via inhibition of NF-κB activation. However, proteasome inhibition could influence the antigen processing and cytotoxic T-cell response and suppress essential immune functions of human CD4+ T cells. It has been shown that bortezomib therapy could increase the rate of opportunistic infectious complications, mainly within eight cycles of treatment during severe lymphocytopenia treatment-induced periods.[13,20] Corticosteroid therapy is widely associated with nocardial infections. Dexamethasone-based regimens in multiple myeloma increase the rate of bacterial infections (mainly encapsulated bacteria such as Staphylococcus aureus, and others including Enterobacteriaceae, Pseudomonas aeruginosa, mycobacteria), viral and micotic infections.[21] An observational study of 13 patients collected over a 13-years period in two Spanish institutions showed that the most common risk factor for both pulmonary and disseminated nocardiosis was corticosteroid therapy (64% in the whole group of patients, 45.5% in disseminated nocardiosis patients).[22] Dose and duration of steroid treatment in nocardiosis patients receiving corticosteroids prior to diagnosis varied widely in that study ranging from prednisone 40 mg/day for 15 days to prednisone 7.5 mg/day for 13 years.
Our patient received for the nocardiosis a combination regimen of TMP-SMX plus meropenem that was switched to a long term oral therapy with ciprofloxacin. Optimal antimicrobial treatment regimens have not been firmly established for nocardiosis, and the management of the disease must be individualized. Immunosuppressed patients and those with CNS disease should receive at least 12 months of antimicrobial therapy with the appropriate clinical monitoring.[7] In CNS nocardiosis, the antibiotic regimen should include drugs with a favorable CNS penetration, as the ones we used for our patients.
In conclusion, this is the first report of multiple brain abscesses caused by N. paucivorans in a patient suffering from multiple myeloma. In this patient the risk factors for a nocardial infection included the hematological disease and chemotherapy.  Among the various chemotherapy regimens, we suggest that corticosteroids and lenalidomide had a pivotal role as risk factors for nocardiosis in the present case. Although nocardial infection itself in multiple myeloma is an uncommon infectious complication so far,[13] nocardial brain abscesses should be added to the spectrum of adverse effect due to the novel anti-myeloma drugs. We emphasize the importance in nocardial infections of obtaining the species identification and the strain susceptibility in order to guide the long term antibiotic therapy.
Finally, in our case etiological diagnosis was made possible by culture of brain biopsy. Other than the nocardial infection, the causes of focal brain infections in hematologic malignancies  could include for instance neurotoxoplasmosis, zygomycosis, aspergillosis, fusariosis.[23] Given the risks of misdiagnosis or delayed diagnosis, we highlight the importance of such invasive procedure, as a brain biopsy, to obtain samples for microbiological analysis in all cases of focal brain lesions, especially in the immunocompromised host.

Contributions, Conflict of Interest and Ethical Statement

All the authors approved the final version of the manuscript. JM reviewed the clinical literature and drafted the first version of the manuscript. RL edited the manuscript and provided intellectual inputs. CF identified the strain and drafted the microbiological section of this report. CM, CR, RV equally treated the patient, collected the clinical data in this report and edited the manuscript. The authors declare that they have no conflict of interest. The patient signed an informed consent form for allowing this case report to be published for scientific purposes. 

Acknowledgements

We would like to thank doctor Gabriele Bazzocchi, Trieste (Italy), for providing the radiological imaging.

References

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