INFLUENCE OF SICKLE CELL GENE ON THE ALLELIC DIVERSITY AT THE MSP-1 LOCUS OF PLASMODIUM FALCIPARUM IN ADULT PATIENTS WITH SEVERE MALARIA

Dilip Kumar Patel, Ranjeet Singh Mashon, Prasanta Purohit, Siris Patel, Satyabrata Meher, Snehadhini Dehury, Chhatray Marndi, Kishalaya Das, Bipin Kishore Kullu, Padmalaya Das
  • Dilip Kumar Patel
    Associate Professor, Department of Medicine, Head, Sickle Cell Clinic and Molecular Biology Laboratory, Project Coordinator, Odisha Sickle Cell Project, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India, India
  • Ranjeet Singh Mashon
    Senior Research Fellow, Sickle Cell Clinic and Molecular Biology Laboratory Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India, India
  • Prasanta Purohit
    Senior Research Fellow Indian Council of Medical Research (ICMR) V.S.S.Medical College, Burla, Odisha, India, India | prasanta.biochem@gmail.com
  • Siris Patel
    Senior Medical Officer, Sickle Cell Clinic and Molecular Biology Laboratory, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India, India
  • Satyabrata Meher
    Research Assistant, Sickle Cell Clinic and Molecular Biology Laboratory, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India., India
  • Snehadhini Dehury
    Research Assistant, Sickle Cell Clinic and Molecular Biology Laboratory, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India., India
  • Chhatray Marndi
    Post Graduate Student of Internal Medicine, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India., India
  • Kishalaya Das
    Scientific Officer, Sickle Cell Clinic and Molecular Biology Laboratory Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India., India
  • Bipin Kishore Kullu
    Assistant Professor, Department of Medicine, Veer Surendra Sai Medical College, Burla, Sambalpur, Odisha, India., India
  • Padmalaya Das
    Senior Scientist, Department of Infectious Diseases, Asian Institute of Public Health, Bhubaneswar, Odisha, India, India

Abstract

Although several studies have supported that sickle cell trait (HbAS) protects against falciparum malaria, the exact mechanism by which sickle gene confers protection is unclear. Further, there is no information on the influence of sickle gene on parasitic diversity of P. falciparum population in severe symptomatic malaria.  This study was undertaken to assess the effect of the sickle gene on the parasite densities and diversities in hospitalized adult patients with severe falciparum malaria. The study was carried out in 166 adult hospitalized subjects with severe falciparum malaria at Sickle Cell Clinic and Molecular Biology Laboratory, Veer Surendra Sai Institute of Medical Sciences and Research, Burla, Odisha, India . They were divided into three groups on the basis of hemoglobin variants HbAA (n=104), HbAS (n=30) and HbSS (n=32). The msp-1 loci was genotyped using a PCR based methodology. The parasite densities were significantly high in HbAA compared to HbAS and HbSS. The multiplicity of infection (MOI) and multiclonicity for msp-1 were significantly low in HbSS and HbAS compared to HbAA. The prevalence of K1 (p<0 .0001) and MAD20 (p=0.0003) alleles were significantly high in HbAA. The RO33 allele was detected at a higher frequency in HbSS and HbAS, compared to K1 and MAD20. Sickle gene was found to reduce both the parasite densities and diversity of P. falciparum in adults with severe malaria.

Keywords

Sickle Gene; Malaria; msp-1; Plasmodium falciparum; Severe; Adult

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