Makis

VCAM-1 and ICAM-1 Serum Levels as Markers of Relapse in Visceral Leishmaniasis 

Alexandros Makis, Sophia Tsabouri, Paraskevi Karagouni, Maria Rogalidou, Irene Sionti and Nikolaos Chaliasos

Child Health Department, Faculty of Medicine, University of Ioannina, Ioannina, Greece.

Corresponding author: Alexandros Makis, Assistant Professor of Pediatrics/Pediatric Hematology, Child Health Department, Faculty of Medicine, University of Ioannina, P.O. Box  1187, GR-45110 Ioannina, Greece. Tel: +30 2651099598, Fax: +30 2651007038. E-mail: amakis@cc.uoi.gr 

Published: January 1, 2017
Received: October 3, 2016
Accepted: November 18, 2016
Mediterr J Hematol Infect Dis 2017, 9(1): e2017011 DOI 10.4084/MJHID.2017.011
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Visceral leishmaniasis (VL), caused by the protozoan parasite Leishmania donovani infection, is characterized by a varied spectrum of clinical and laboratory manifestations and by the potentiality of relapses (1,5-10%) despite current therapy.[1] Following infection, many cell adhesion interactions have been identified among monocytes/macrophages, vascular endothelial cells and the parasite;[2,3] and various changes in the expression of adhesion molecules VCAM-1 (vascular cell adhesion molecule-1), ICAM-1 (intracellular adhesion molecule-1) and L-selectin have been found in experimental VL.[4,5] We hypothesized that the monitoring of this cell to cell interaction system through the course of VL could be useful in estimating the disease progress.
We enrolled in the study 16 children (10 boys, 2-15 years) hospitalized for VL. Most of the children had presented with fever, hepatosplenomegaly, anemia and thrombocytopenia. The clinical diagnosis was confirmed by positive serology, PCR technology or parasite presence in the bone marrow macrophages (Table 1). The standard treatment regimen was liposomal amphotericin B (3 mg/kg) on days 1 to 5, 14, and 21. The serum levels of VCAM-1, ICAM-1, and L-selectin were determined in the patients at days 0, 15 and 30, as well as in 20 gender and age-matched healthy children. Commercial ELISA kits were used (Quantikine, R&D Systems, Inc., Minneapolis, USA). Mann-Whitney U test, Wilcoxon matched pairs test and x2 test were appropriately used for the statistical analysis.


Table 1 Table 1. Clinical and laboratory characteristics in 16 children with visceral leishmaniasis at diagnosis.

All children recovered completely, while three children relapsed 3, 5 and 6 months after treatment. At day 0, VCAM-1, ICAM-1, and L- selectin were similar to controls (p>0.05). At day 15, VCAM-1 and ICAM-1 were significantly increased (P=0.0012, P=0.0032) where L-selectin remained stable (P=0.75). At day 30, VCAM-1 and ICAM-1 decreased at levels comparable to pretreatment values in the 13 children who subsequently had a good outcome without relapses (P=0.88), but not in the three patients who relapsed (P=0.0007). No differences were noted in L-selectin levels (P=0.19) (Table 2). The adhesion molecules levels were further analyzed for both non-relapsers and relapsers. Non-relapsers showed a significant decline in VCAM-1 and ICAM-1 levels at day 30 (P=0.0006 and P=0.0008) compared to day 15. By contrast, in relapsers day 30 serum VCAM-1 and ICAM-1 had not significantly decreased as compared to day 15 (P=0.45, P=0,72). No differences were demonstrated on day 0, 15 and 30 L-selectin values (Table 3). No differences were noted regarding gender, age, symptoms and the laboratory tests on admission, such as hemoglobin, white blood cell counts, platelets, C-reactive protein, erythrocyte sedimentation rate, total protein levels or albumin levels.

Table 2 Table 2. Serum levels of VCAM-1, ICAM-1 and L-selectin (mean ± standard error of the mean) in children with visceral leishmaniasis and controls.

Table 3 Table 3. Serum levels of VCAM-1, ICAM-1 and L-selectin (mean ± standard error of the mean) during treatment in relapsers and non-relapsers.

These findings may be explained by the cell adhesion interactions during the immune response and the effect of the anti-parasite treatment. The suppressed VCAM-1 and ICAM-1 levels at diagnosis could reflect the adverse effect of Leishmania against the adhesive interactions to stop the leukocyte attraction to the site of parasitic infection. The interaction of VCAM-1 with its ligands is crucial for the efficient control of Leishmania donovani infection, especially in the liver. Interestingly, the blockade of VCAM-1 leads suppresses anti-leishmania immune responses and leads to higher hepatic parasite accumulation.[6] A similar mechanism of down-regulation of ICAM-1 has been found in human synovial cells in vitro infected with Borrelia burgdorferi.[7] The elevation of VCAM-1 and ICAM-1 in day 15 could be the beneficial effect of liposomal amphotericin B which destroys the parasites and allows the cell to cell interactions. After the end of treatment, the number of tissue parasites dramatically diminishes, and this is probably the reason why the VCAM-1 and ICAM-1 levels return to pre-treatment levels in the children who had a good long-term outcome without relapses. The persistence of high VCAM-1 and ICAM-1 values in the children who relapsed despite they received the same treatment possibly reflects the ongoing immune response to the remaining parasites as well as the action of liposomal amphotericin B. Maybe, these children had a tissue parasite burden larger at front, and they could have taken advantage from the repetition of second treatment schedule. L-selectin did not show any alterations during the disease. One possible explanation is that L-selectin acts on lymphocyte-endothelial cell interactions and activates Th2 (T helper 2) immune response, which is not implicated in the host defense against Leishmania.[8]
In conclusion, we found that serum levels of VCAM-1 and ICAM-1 at day 30 post-treatment demonstrated statistically significant correlation with the possibility to relapse in this small group of patients, while L-selectin showed no association. Despite the low number of the patients of this study, our findings indicate that the measurement of VCAM-1 and ICAM-1 during the course of VL may guide and predict disease evolution and outcome in children. Although the mechanisms underlying the association between serum VCAM-1 and ICAM-1 levels and the adverse outcome has not yet been elucidated, further investigation with a larger number of patients would clarify their role as factors of disease severity and confirm their importance as prognostic markers.

References

  1. Georgiadou SP, Stefos A, Spanakos G, Skrimpas S, Makaritsis K, Sipsas NV, et al. Current clinical, laboratory, and treatment outcome characteristics of visceral leishmaniasis: results from a seven-year retrospective study in Greece. Int J Infect Dis. 2015;34:46-50. https://doi.org/10.1016/j.ijid.2015.02.021 PMid:25743761   
  2. Rodrigues V, Cordeiro-da-Silva A, Laforge M, Silvestre R, Estaquier J. Regulation of immunity during visceral Leishmania infection. Parasit Vectors. 2016;9:118. https://doi.org/10.1186/s13071-016-1412-x PMid:26932389 PMCid:PMC4774109   
  3. Figueira CP, Carvalhal DG, Almeida RA, Hermida M, Touchard D, Robert P, et al. Leishmania infection modulates beta-1 integrin activation and alters the kinetics of monocyte spreading over fibronectin. Sci Rep. 2015;5:12862. https://doi.org/10.1038/srep12862 PMid:26249106 PMCid:PMC4528201   
  4. Engwerda CR, Ato M, Stager S, Alexander CE, Stanley AC, Kaye PM. Distinct roles for lymphotoxin-alpha and tumor necrosis factor in the control of Leishmania donovani infection. Am J Pathol. 2004;165(6):2123-33. https://doi.org/10.1016/S0002-9440(10)63262-2    
  5. Colpitts SL, Scott P. The early generation of a heterogeneous CD4+ T cell response to Leishmania major. J Immunol. 2010;185(4):2416-23. https://doi.org/10.4049/jimmunol.1000483 PMid:20624946 PMCid:PMC2944829  
  6. Stanley AC, Dalton JE, Rossotti SH, MacDonald KP, Zhou Y, Rivera F, et al. VCAM-1 and VLA-4 modulate dendritic cell IL-12p40 production in experimental visceral leishmaniasis. PLoS Pathog. 2008;4(9):e1000158. https://doi.org/10.1371/journal.ppat.1000158 PMid:18802456 PMCid:PMC2528938   
  7. Girschick HJ, Meister S, Karch H, Huppertz HI. Borrelia burgdorferi downregulates ICAM-1 on human synovial cells in vitro. Cell Adhes Commun. 1999;7(2):73-83. https://doi.org/10.3109/15419069909034398 PMid:10427961      
  8. Seixas Duarte MI, Tuon FF, Pagliari C, Kauffman MR, Brasil RA. Human visceral leishmaniasis expresses Th1 pattern in situ liver lesions. J Infect. 2008;57(4):332-7. https://doi.org/10.1016/j.jinf.2008.07.005 PMid:18722018      
      

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