Eleonora De Bellis1, Luana Fianchi2, Francesco Buccisano1, Marianna Criscuolo2, Luca Maurillo1, Laura Cicconi1, Mattia Brescini1, Maria Ilaria Del Principe1, Ambra Di Veroli1, Adriano Venditti1, Sergio Amadori1, William Arcese1, Francesco Lo-Coco1 and Maria Teresa Voso1
Received: April 30, 2017
Accepted: June 10, 2017
Mediterr J Hematol Infect Dis 2017, 9(1): e2017045 DOI 10.4084/MJHID.2017.045
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2002, the WHO classification reduced the proportion of blasts in the
bone marrow (BM) necessary for the diagnosis of acute myeloid leukemia
(AML) from 30% to 20%, eliminating the RAEB-t subtype of
myelodysplastic syndromes (MDS). However, this AML subtype, defined as
low-blast count AML (LBC-AML, with 20-30% BM-blasts) is characterized
by peculiar features, as increased frequency in elderly individuals and
after cytotoxic treatment for a different primary disease
(therapy-related), poor-risk cytogenetics, lower white blood cell
counts, and less frequent mutations of NPM1 and FLT3
genes. The clinical course of this entity is often similar to MDS with
10-19% BM-blasts. The hypomethylating agents azacitidine and decitabine
have been shown to induce responses and prolong survival both in MDS
and LBC-AML.The role of these agents has also been demonstrated in AML
with >30% BM-blasts, particularly in patients with poor-risk
cytogenetics and in AML with myelodysplasia-related changes. Most
recent studies are evaluating strategies to improve outcome, including
combinations of hypomethylating agents with immune-response checkpoint
inhibitors, which have a role in cancer immune surveillance. Efforts
are also ongoing to identify mutations which may predict response and
survival in these patients.
Despite the newer WHO classifications, the international prognostic scoring system (IPSS) has still been used for many years to stratify patients with MDS in two prognostic risk groups (low/Int-1 and Int-2/high, defined as lower- and higher-risk MDS, respectively). The original IPSS score included RAEB-t (20-29% blasts), and this led to the inclusion of this patient subset in MDS protocols, in particular when using hypomethylating treatment (HMT). Most recently, the IPSS-Revised has been introduced, and this scoring system only classifies patients with MDS, up-to 19% BM blasts.
The purpose of this review is to summarize most recent evidence on the outcome of LBC-AML, taking into account the introduction of HMT, and improved supportive care measures.
In 2014, the AGMT-Study Group reported on efficacy and safety of azacitidine in a cohort of 302 AML patients including both patients with 20-29% and ≥30% BM-blasts, who had received at least one dose of azacitidine. Overall response rate (ORR) was 48% in the total cohort, and 72% in patients evaluable according to MDS-IWG-2006 response criteria (after at least 2 AZA cycles), respectively. Median time to first response was 3.0 months: this corresponded to the best response in 69% of cases, though the median duration of response was 3.4 months (range 0.3-33.0). As a significant result, patients who achieved hematological improvement (HI platelets, and/or neutrophils, and/or erythrocytes) had significantly longer OS than those who did not (16.1 vs. 4.5 months, p=<0.001). This underlines the importance to continue HMT in the case of HI, regardless of bone marrow response. On the other hand, BM-blast counts did not significantly affect OS, both in the whole patient cohort and after excluding pre-treated patients.
The international phase III AZA-AML-001 study was the first prospective, randomized study to evaluate efficacy and safety of azacitidine compared with CCR (BSC only, LD-cytarabine, or standard IC) in elderly patients (age ≥65 years), with newly diagnosed AML and >30% BM blasts. Inclusion criteria included ineligibility for allogeneic stem cell transplantation (HSCT), intermediate- or poor-risk cytogenetics (NCCN 2009 criteria), ECOG ≤ 2 and white blood cell counts ≤15 × 109/L. A total of 481 patients were randomized (AZA n=241, CCR n=240). Median OS for patients receiving AZA and CCR was 10.4 and 6.5 months, respectively; stratified HR was 0.85 (95% CI, 0.69-1.03; p=0.1009). The survival benefit became significant in the pre-planned post-hoc Cox model, evaluating the time to subsequent treatment. The risk of death was reduced by 25% in the AZA arm, and median OS was prolonged by more than 5 months, compared with CCR (12.1 vs 6.9 months; stratified HR, 0.76; 95% CI, 0.60-0.96; p=0.0190). Univariate OS analyses documented favorable trends for AZA therapy compared with CCR across all subgroups, with a statistically significant survival benefit in patients with poor-risk cytogenetics and in AML with myelodysplasia related changes, consistent with the positive results previously reported by several groups in patients with higher-risk MDS. No significant OS improvement was observed in therapy-related AML, but there was a positive trend for the azacitidine-treated group.
Therapy-related myeloid neoplasms (t-MN), including myelodysplastic syndromes and acute myeloid leukemia (t-MDS and t-AML) are associated to clinical and biologic unfavorable prognostic features, including changes in DNA methylation levels. Due to the association with exposure to DNA-damaging agents, including chemo- and radiotherapy, and the possible common pathways of leukemic transformation, these diseases have been grouped together and included in the WHO classification of AML since 2002.[1,9] A multicenter retrospective study was conducted by our group in 50 patients (34 t-MDS and 16 t-AML) that received azacitidine as induction treatment. Overall response rate was 42% (CR: 21%, Partial Remission, PR: 4.2% and HI: 16.7%) and was obtained after a median of 3 cycles (range 1–6). Stable disease (SD) was documented in 31% of patients. Median overall survival was 21 months (range 1–53.6+) from azacitidine start, and was significantly better in patients with BM blasts <20% and in t-AML patients with normal karyotype, consistent with the known important prognostic role of cytogenetics. Comparing the efficacy of azacitidine in 196 de novo MDS/LBC AML, vs 58 t-MN, we did not observe any survival differences (median 16.9 vs 16.2 months, respectively, p= 0.1997), sustaining the activity of AZA in the t-MN setting, independent of the previous history of cytotoxic treatment (Fianchi & Voso, unpublished).
Recently, the efficacy of azacitidine was compared with that of intensive chemotherapy in elderly patients with AML secondary to a previous MDS, myeloproliferative neoplasm, or prior cytotoxic exposure. t-AML accounted for 45% of cases. Median BM-blast count was 30% (range 25-62) and 50% (range 27-82) in the azacitidine and IC groups, respectively (p<0.0001). In this study, there was no significant survival difference comparing chemotherapy and azacitidine (9.6 vs 10.8 months, respectively, p = 0.899). Adjusted time-dependent analyses showed that survival was indeed similar up to 1.6 years post-treatment. After this time-point, patients who received chemotherapy had a lower risk of death compared to those who received azacitidine (adjusted HR 0.61, 95%CI: 0.38-0.99, at 1.6 years).
An international, multicenter, randomized, open-label, phase III trial conducted by Kantarjian et al., compared efficacy and safety of decitabine with physician’s treatment choice (LD-cytarabine or best supportive care) in a cohort of 485 elderly patients, of a median age of 73 years (64-91), with newly diagnosed de novo or secondary AML, and poor- or intermediate-risk cytogenetics. The decitabine schedule was 20 mg/m2 per day as a 1-hour intravenous infusion, for five consecutive days, every 4 weeks. Significantly improved remission rates were observed with decitabine versus physician’s treatment choice, with 17.8% CR or CRp (CR with incomplete platelet recovery), vs 7.8%; respectively (P = 0.001). Despite the fact that the survival difference was not significant at the 2009 cut-off year, mature survival data collected in 2010 showed that there was a significantly improved OS for patients treated with decitabine (nominal p = 0.037). The trend towards a benefit for decitabine treatment was more clearly observed in patients ≥ 70 years old, with de novo AML, over 30% baseline BM-blasts, intermediate- or poor-risk cytogenetics, and ECOG PS 2, versus 0 to 1. Similar results have been reported by Bhatnagar et al. in a retrospective analysis on 45 previously untreated AML patients, judged unfit for intensive chemotherapy, and treated with a 10 day-decitabine schedule. The ORR was 42%, with 31% CR, and 11% CR with incomplete count recovery. The response rate was higher in patients with lower pre-treatment BM-blasts counts (42%), as compared to patients with higher BM-blasts (p = 0.01).
To try to increase treatment efficacy, a 10-day DAC schedule was explored in a phase II clinical trial using single-agent decitabine, in patients aged over 60 years, with previously untreated AML. The ORR was 64%, including 47% CR and 17% CR with incomplete count recovery, with no difference according to karyotype. This pilot study showed promising results in terms of response and overall survival in elderly AML patients treated with the prolonged decitabine-schedule, regardless of blast count at diagnosis. Another important result was the demonstration that toxicity was similar to the 5-day schedule.
Similar data were reported by Ritchie et al., who treated 52 patients using the 10-day Decitabine schedule, for at least one induction cycle. After achieving CR, most patients continued with the 5-day schedule, until toxicity or disease progression. The CR rate was 46% and the median OS was 318 days, while the median number of cycles required to achieve a response was 2 (1-4 cycles). Also in this study, the 10-day schedule was well tolerated, with toxicities similar to the 5-day schedule.
The combination of HMT with immune-checkpoint inhibitors (ICI) is a promising approach. The PD-1 pathway has a role in immune surveillance and is composed by a co-stimulatory receptor primarily expressed on activated T-cells (PD-1), and its ligands, that are primarly expressed on tumor cell surface (PD-L1 and PD-L2). Binding of PD-1 to its ligands PD-L1 and PD-L2 inhibits effector T-cell function and this interaction can suppress immune surveillance and permit neoplastic growth.[20,21] Evaluation of expression of PD-1 pathway proteins in patients with myeloid neoplasms showed increased expression of PD-L1, PD-L2, PD-1 and cytotoxic T-lymphocyte-associated antigen 4 (CTLA4) in CD34+ peripheral blood cells from patients with MDS, LMMC and AML. Expression of PD-L1 was significantly higher in MDS and CMML, compared to AML. In patients undergoing Decitabine, these genes were upregulated due to demethylation of PD-1, particularly in patients resistant to therapy, compared with those who achieved a hematologic response. This indicates that PD-1 signaling may be involved in MDS pathogenesis and mechanisms of resistance to hypomethylating agents. Ørskov et al. identified a correlation between PD-1 promoter demethylation and increased PD-1 expression in PB T-cells of patients with MDS, following consecutive cycles of AZA, that resulted into significantly worse ORR (8% vs. 60%, p = 0.014), and shorter OS (p = 0.11). A significantly higher baseline methylation level of the PD-1 promoter was observed in T-cells of non-responding patients also when compared to healthy controls (p = 0.023). The HMT/ICI combination may increase treatment response due to demethylation and re-activation of genes related to interferon signaling, antigen presentation and inflammation, which may favour the activity of ICI.
Another potential target, shown to be overexpressed in CD34+ cells of patients with AML, is the anti-apoptotic protein B-cell lymphoma 2 (BCL-2), which plays a role in therapy resistance. Venetoclax (ABT-199) is an orally bioavailable, selective BCL-2 inhibitor that has been used in older, newly diagnosed AML patients not eligible for intensive chemotherapy. Treatment combinations of Venetoclax with HMT showed promising results, with 76% ORR in 39 AML patients treated in a phase 1b study.
New Hypomethylating Agents
The oral formulation of azacitidine CC-486 may also represent an effective alternative approach to patients with MDS, CMML and AML. CC-486 was evaluated in 3 phase 1-2 studies, including patients who had previously received standard HMT (50% of patients with AML). Five of 13 patients (38%) refractory to prior HMT responded, including 1 patient with AML who achieved CR. The ORR was 35% and no significant difference in ORR and in the rate of specific responses (CR, PR, CRi, HI and transfusion independence) was observed between patients with MDS, CMML or AML. Similar response rates were achieved in patients who relapsed or were refractory to prior HMT, suggesting that HMT failure does not preclude future response to CC-486.
Prognostic Factors for HMT in AML
Research currently aims at identifying somatic mutations that could be useful to predict response to HMT. Bejar et al. sequenced 40 genes recurrently mutated in myeloid malignancies in the BM-DNA from 213 MDS patients collected before treatment with azacitidine or decitabine. The overall response rate of 47% was not different between agents. None of the mutations was predictive of response per se, but TET2 mutations predicted a significantly higher response rate to HMT (when at over 10% variant allele fraction), compared to wild-type TET2. Response rates were highest in the subset of TET2-mutant patients without clonal ASXL1 mutations (OR 3.65, P = .009). On the other hand, mutations of TP53 were a negative predictor of survival (P= .002) and identified a particularly poor prognostic subgroup in patients with complex karyotype, with a median survival of only 0.9 years, compared to 1.3 years in patients with complex cytogenetics and no TP53 mutations (P= .003). In this last subgroup, survival was not different from that of patients with other karyotype abnormalities (median 1.8 years, P = .28). This suggests that the adverse prognostic value ascribed to complex karyotype is largely induced by its frequent association with TP53 mutations, also during HMT. These data partially contrast with more recent reports on the prognostic role of TP53 mutations in the context of HMT. Muller-Thomas et al., showed that TP53-mutated patients had a higher probability of response to AZA, compared to TP53-WT patients. This difference was more pronounced in MDS.
This has been confirmed by a recent paper by Welch et al., in patients with MDS or AML treated with 20 mg/m2 Decitabine for 10 days. Response rates were higher in patients with unfavorable cytogenetics than in patients with intermediate-risk or favorable-risk cytogenetics (67% vs. 34%, P<0.001), and in TP53-mutated, compared to TP53-WT patients (21 of 21 [100%] vs. 32 of 78 [41%], P<0.001). Furthermore, TP53-mutated allelic burden significantly decreased after four serial 10-day courses of decitabine. Although responses were not durable, overall survival rates of TP53-mutated patients were similar to those of patients with intermediate-risk AML, who received the same treatment schedule. These data indicate that HMT may significantly modify the prognostic impact of adverse genetic alterations, particularly TP53 mutations. However, these data need to be confirmed in larger, prospective studies.
- Vardiman JM, Harris NL, Brunning RD. The World
Health Organization (WHO) classification of myeloid neoplasms. Blood
2002; 100:2292-2302. https://doi.org/10.1182/blood-2002-04-1199
- Di Nardo CD, Garcia-Manero G, Pierce S, Nazha A, Bueso-Ramos C, Jabbour E, Ravandi F, Cortes J and Kantarjian H. Interactions and relevance of blast percentage and treatment strategy among younger and older patients with acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). Am J Hematol 2016; 91:227–232. PMID: 26799610 https://doi.org/10.1002/ajh.24252
- Greenberg P, Cox C, LeBeaux MM, Fenaux P, Morel P, Sanz G, Sanz M, Vallespi T, Hamblin T, Oscier D, Ohyashiki K, Toyama K, Aul C, Mufti G and Bennett J. International scoring system for evaluating prognosis in myelodysplastic syndromes. Blood 1997; 89:2079-2088. PMID: 9058730
- Greenberg PL, Tuechler H, Schanz J, Sanz G, Garcia-Manero G, Solé F, Bennett JM, Bowen D, Fenaux P, Dreyfus F, Kantarjian H, Kuendgen A, Levis A, Malcovati L, Cazzola M, Cermak J, Fonatsch C, Le Beau MM, Slovak ML, Krieger O, Luebbert M, Maciejewski J, Magalhaes SMM, Miyazaki Y, Pfeilstöcker M, Sekeres M, Sperr WR, Stauder R, Tauro S, Valent P, Vallespi T, van de Loosdrecht AA, Germing U and Haase D. Revised international prognostic scoring system for myelodysplastic syndromes. Blood 2012; 120:2454-2465. https://doi.org/10.1182/blood-2012-03-420489 PMCID: PMC4425443.
- Fenaux P, Mufti GJ, Hellstrom-Lindberg E, Santini V, Finelli C, Giagounidis A, Schoch R, Gattermann R, Sanz G, List A, Gore SD, Seymour JF, Bennett JM, Byrd J, Backstrom J, Zimmerman L, McKenzie D, Beach CL and Silverman LR. Efficacy of Azacitidine compared with that of conventional care regimens in the treatment of higher-risk myelodysplastic syndromes: a randomised, open-label, phase III study. Lancet Oncol 2009; 10:223-232. PMCID: PMC4086808. https://doi.org/10.1016/S1470-2045(09)70003-8
- Fenaux P, Mufti GJ, Hellstrom-Lindberg E, Santini V, Gattermann N, Germing U, Sanz G, List AF, Gore S, Seymour JF, Dombret H, Backstrom J, Zimmerman L, McKenzie D, Beach CL and Silverman LR. Azacitidine prolongs overall survival compared with conventional care regimens in elderly patients with low bone marrow blast count acute myeloid leukemia. J Clin Oncol 2010; 28:562-569. https://doi.org//10.1200/JCO.2009.23.8329 PMID: 20026804.
L, Burgstaller S, Girschikofsky M, Linkesch W, Stauder R, Pfeilstocker
M, Schreder M, Tinchon C, Sliwa T, Lang A, Sperr WR, Krippl P, Geissler
D, Voskova D, Schlick K, Thaler J, Machherndl-Spandl S, Theiler G,
Eckmüllner O and Greil R. Azacitidine in 302 patients with WHO-defined
acute myeloid leukemia: results from the Austrian Azacitidine Registry
of the AGMT-Study Group. Ann Hematol 2014; 93:1825-1838. DOI https://doi.org/10.1007/s00277-014-2126-9 PMCID: PMC4176957 .
- Dombret H, Seymour JF, Butrym A. Wierzbowska A, Selleslag D, Jang JH, Kumar R, Cavenagh J, Schuh AC, Candoni A, Récher C, Sandhu I, Bernal del Castillo T, Al-Ali HK, Martinelli G, Falantes J, Noppeney R, Stone R, Minden MD, McIntyre H, Songer S, Lucy LM, Beach CL and Döhner H. International phase 3 study of azacitidine vs conventional care regimens in older patients with newly diagnosed AML with >30% blasts. Blood 2015; 126:291-299. https://doi.org/10.1182/blood-2015-01-621664 PMID: 25987659.
- Arber DA, Orazi A, Hasserjian R, Thiele J, Borowitz MJ, Le Beau MM, Bloomfield CD, Cazzola M and Vardiman JW. The 2016 revision to the World Health Organization classification of myeloid neoplasms and acute leukemia. Blood 2016; 127:2391-2405. https://doi.org/10.1182/blood-2016-03-643544 PMID: 27069254.
- Fianchi L, Criscuolo M, Lunghi M, Gaidano G, Breccia M, Levis A, Finelli C, Santini V, Musto P, EN Oliva, Leoni P, Spiriti AA, D Alò F, Hohaus S, Pagano L, Leone G and Voso MT. Outcome of therapy-related myeloid neoplasms treated with azacitidine. J Hematol Oncol 2012; 5:44. https://doi.org/10.1186/1756-8722-5-44
- Dumas PY, Bertoli S, Bérard E, Médiavilla C, Yon E, Tavitian S, Leguay T, Huguet F, Forcade F, Milpied N, Sarry A, Sauvezie M, Bories P, Pigneux A and Récher C. Azacitidine or intensive chemotherapy for older patients with secondary or therapy-related acute myeloid leukemia. Oncotarget 2017. http://www.impactjournals.com/oncotarget
- Kantarjian H1, Issa JP, Rosenfeld CS, Bennett JM, Albitar M, DiPersio J, Klimek V, Slack J, de Castro C, Ravandi F, Helmer R 3rd, Shen L, Nimer SD, Leavitt R, Raza A, Saba H. Decitabine improves patient outcomes in myelodysplastic syndromes: results of a phase III randomized study. Cancer 2006; 106:1794-1803. DOI: 10.1002/cncr.21792. https://doi.org/10.1002/cncr.21792
- Kantarjian HM, Thomas XG, Dmoszynska A, Wierzbowska A, Mazur G, Mayer G, Gau J-P, Chou W-C, Buckstein R, Cermak J, Kuo C-Y, Oriol A, Ravandi F, Faderl S, Delaunay J, Lysa D, Minden M and Arthur C. Multicenter, randomized, open-label, phase III trial of decitabine versus patient choice, with physician advice, of either supportive care or low-dose cytarabine for the treatment of older patients with newly diagnosed acute myeloid leukemia. Clin Oncol 2012; 30:2670-2677. PMID: 22689805. https://doi.org/10.1200/JCO.2011.38.9429
- Bhatnagar B, Duong VH, Gourdin TS, Tidwell ML, Chen C, Ning Y, Emadi A, Sausville EA and Baer MR. Ten-day decitabine as initial therapy for newly diagnosed patients with acute myeloid leukemia unfit for intensive chemotherapy. Leuk Lymphoma 2014; 55:1533-1537. PMID: 24144313. https://doi.org/10.3109/10428194.2013.856425
- Blum W, Garzon R, Klisovic RB, Schwind S, Walker A, Geyer S, Liu S, Havelange V, Becker H, Schaaf L, Mickle J, Devine H, Kefauver C, Devine SM, Chan KK, Heerema NA, Bloomfield CD, Grever MR, Byrd JC, Villalone-Calero M, Croce CM, Marcucci G. Clinical response and miR-29b predictive significance in older AML patients treated with a 10-day schedule of decitabine. Proc Natl Acad Sci USA 2010; 107:7473-7478. https://doi.org/10.1073/pnas.1002650107
- Ritchie EK, Feldman EJ, Christos PJ, Rohan SD, Lagassa CB, Ippoliti C, Scandura JM, Carlson K, Roboz GJ. Decitabine in patients with newly diagnosed and relapsed acute myeloid leukemia. Leuk Lymphoma 2013; 54:2003-2007. https://doi.org/10.3109/10428194.2012.762093
- Prebet T, Sun Z, Figueroa ME, Ketterling R, Melnick A, Greenberg PL, Herman J, Juckett M, Smith MR, Malick L, Paietta E, Czader M, Litzow M, Gabrilove J, Erba HP, Gore SD and Tallman MS. Prolonged Administration of Azacitidine With or Without Entinostat for Myelodysplastic Syndrome and Acute Myeloid Leukemia With Myelodysplasia-Related Changes: Results of the US Leukemia Intergroup Trial E1905 J Clin Oncol 2014; 12:1242-1249. PMID: 24663049. https://doi.org/10.1200/JCO.2013.50.3102
- Zhao WH, Zeng QC, Huang BT, Li BS and Chen RL. Decitabine plus thalidomide yields more sustained survival rates than decitabine monotherapy for risk-tailored elderly patients with myelodysplastic syndrome. Leuk Res 2015; 39:424-428. PMID: 25721158. https://doi.org/10.1016/j.leukres.2015.01.014
- Boussiotis VA. Molecular and Biochemical Aspects of the PD-1 Checkpoint Pathway. N Engl J Med 2016; 375:1767-1778. https://doi.org/10.1056/NEJMra1514296
- Blank C, Gajewski TF and Mackensen A. Interaction of PD-L1 on tumor cells with PD-1 on tumor-specific T cells as a mechanism of immune evasion: implications for tumor immunotherapy. Cancer Immunol Immunother 2005; 54:307-314. PMID: 15599732. https://doi.org/10.1007/s00262-004-0593-x
- Pardoll DM. The blockade of immune checkpoints in cancer immunotherapy. Nat Rev Cancer 2012; 12:252-264. PMID: 22437870. https://doi.org/10.1038/nrc3239
- Yang H, Bueso-Ramos C, Di Nardo C, Estecio MR, Davanlou M, Geng Q-R, Fang Z, Nguyen M, Pierce S, Wei Y, Parmar S, Cortes J, Kantarjian H and Garcia-Manero G. Expression of PD-L1, PD-L2, PD-1 and CTLA4 in myelodysplastic syndromes is enhanced by treatment with hypomethylating agents. Leukemia 2014; 28: 1280-1288. PMID: 24270737. https://doi.org/10.1038/leu.2013.355
- Ørskov AD, Treppendahl MB, Skovbo A, Holm MS, Friis LS, Hokland M and Grønbæk K. Hypomethylation and up-regulation of PD-1 in T cells by azacytidine in MDS/AML patients: A rationale for combined targeting of PD-1 and DNA methylation. Oncotarget 2015; 6:9612–9626. PMID: 25823822. https://doi.org/10.18632/oncotarget.3324
- Wolff F, Leisch M, Greil R, Risch A, Pleyer L. The double-edged sword of (re)expression of genes by hypomethylating agents: from viral mimicry to exploitation as priming agents for targeted immune checkpoint modulation. Cell Commun Signal. 2017; 15: 13. https://doi.org/10.1186/s12964-017-0168-z
- Cang S, Iragavarapu C, Savooji J, Song Y and Liu D. ABT-199 (venetoclax) and BCL-2 inhibitors in clinical development. J Hematol Oncol 2015; 8:129. doi: 10.1186/s13045-015-0224-3. https://doi.org/10.1186/s13045-015-0224-3
- Pollyea DA DC, Thirman MJ, Letai A, Wei AH, Jonas BA, Arellano ML, Frattini MG, Kantarjian HM, Chyla B, Zhu M, Potluri J, Humerickhouse R, Mabry MH, Konopleva M, Pratz KW. Results of a phase 1b study of venetoclax plus decitabine or azacitidine in untreated acute myeloid leukemia patients = 65 years ineligible for standard induction therapy. J Clin Oncol 2016; suppl; abstract 7009.
M, Bally C, Peterlin P, Beyne-Rauzy O, Legros L, Gourin MP, Sanhes L,
Wattel E, Gyan E, Park S, Stamatoullas A, Banos A, Laribi K, Jueliger
S, Bevan L, Chaffaut C, Sapena R, Samey B, Chermat F, Chevret S, Ades L
and Fenaux P. Results of a phase II study of Guadecitabine (SGI-110) in
higher risk MDS, CMML or Low Blast Count AML patients refractory to or
relapsing after Azacitidine (AZA) treatment. Blood 2016; 128:347 .
- Garcia-Manero G, Savona MR, Gore SD, Scott BL, Cogle CR, Boyd T, Conkling P, Hetzer J, Dong Q, Kumar K, Ukrainskyj SM and Skikne BS. CC-486 (Oral Azacitidine) in patients with hematological malignancies who had received prior treatment with injectable Hypomethylating Agents (HMAs): results from phase 1/2 CC-486 studies. Blood 2016;128:905.
- Bejar R, Lord A, Stevenson K, Bar-Natan M, Pérez-Ladaga A, Zaneveld J, Wang H, Caughey B, Stojanov P, Getz G, Garcia-Manero G, Kantarjian H, Chen R, Stone RM, Neuberg D, Steensma DP and Ebert BL. TET2 mutations predict response to hypomethylating agents in myelodysplastic syndrome patients. Blood 2014; 124:2705–2712. doi: https://doi.org/10.1182/blood-2014-06-582809 PMID: 25224413.
- Müller-Thomas C, Rudelius M, Rondak IC, Haferlach T, Schanz J, Huberle C, Schmidt B, Blaser R, Kremer M, Peschel C, Germing U, Platzbecker U and Götze K. Response to azacitidine is independent of p53 expression in higher-risk myelodysplastic syndromes and secondary acute myeloid leukemia. Haematologica 2014; 99:e179-81. PMID: 24972774. https://doi.org/10.3324/haematol.2014.104760
- Welch JS, Petti A, Miller CA, Fronick CC, O'Laughlin M, Fulton RS, Wilson RK, Baty JD, Duncavage EJ, Tandon B, Lee Y-S, Wartman LD, Uy GL, Ghobadi A, Tomasson MH, Pusic I, Romee R, Fehniger TA, Stockerl-Goldstein KE, Vij R, Oh ST, Abboud CN, Cashen AF, Schroeder MA, Jacoby MA, Heath SE, Luber K, Janke MR, Hantel A, Khan N, Sukhanova MJ, Knoebel RW, Stock W, Graubert TA, Walter MJ, Westervelt P, Link DC, DiPersio JF and Ley TJ. TP53 and Decitabine in Acute Myeloid Leukemia and Myelodysplastic Syndromes. N Engl J Med 2016; 375:2023-2036. DOI: 10.1056/NEJMoa1605949. PMID: 27959731. https://doi.org/10.1056/NEJMoa1605949
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