FEASIBILITY OF COLLECTING UMBILICAL CORD BLOOD IN JORDAN AND THE EFFECT OF MATERNAL AND NEONATAL FACTORS ON HEMATOPOIETIC STEM CELL CONTENT

Main Article Content

Ayad Ahmed Hussein *
Randa M. Bawadi
Lubna H. Tahtamouni
Haydar Frangoul
Ali Z. ElKarmi
(*) Corresponding Author:
Ayad Ahmed Hussein | aahmed@khcc.jo

Abstract

Abstract


Background: Cord blood transplant is an accepted treatment for many malignant and non-malignant diseases. We sought to determine the feasibility of collecting cord blood in Jordan and the effect of maternal and fetal factors on the quality of the cord blood units.

Methods: A total of 124 cord blood units were collected and 75 (60%) cord blood units were included in this analysis. Cord blood volume, total nucleated cell (TNC) count, cell viability and CD34+ content were measured, and clonogenic assay was performed.

Results: The mean volume of the collected units was 68.9 ml (range 40-115) with mean nucleated cell count of 6.5 x 108 (range 1-23.0). Our results showed a positive correlation between the volume of cord blood and TNC count (p=0.008), cell viability (p=0.001), CD34+ content (p=0.034) and the length of the umbilical cord (p=0.011). In addition, our results showed an inverse relation between the Colony Forming Unit-Granulocyte Macrophage (CFU-GM) concentration and the gestation duration (p=0.038).

Conclusion: We conclude that it is feasible to collect cord blood units in Jordan with excellent TNC and CD34+ cell content. The volume of cord blood collected was associated with higher TNC count and CD34+ count. Efforts toward establishing public cord blood banks in our area are warranted.

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Author Biographies

Ayad Ahmed Hussein, King Hussein Cancer Center

Head, Pediatric Bone Marrow and Stem Cell Transplant

Randa M. Bawadi, The Hashemite University

Department of Biology and Biotechnology, Faculty of Science

Lubna H. Tahtamouni, The Hashemite University

Department of Biology and Biotechnology, Faculty of Science

Haydar Frangoul, Monroe Carell Jr. Children’s Hospital at Vanderbilt

Pediatric Stem Cell Transplant Program

Ali Z. ElKarmi, The Hashemite University

Department of Biology and Biotechnology, Faculty of Science