Yi-Chang Liu1,2, Ching-Ping Lee1, Tsung-Jang Yeh1, Yuh-Ching Gau1, Chieh-Yu Hsieh1, Ya-Lun Ke1, Jeng-Shiun Du1, Ming-Hui Lin1, Hui-Ching Wang1, Shih-Hao Tang1, Shih-Feng Cho1,2, Chi-En Hsiao1, Jui-Feng Hsu1, Samuel Yien Hsiao4, Chin-Mu Hsu1,3 and Hui-Hua Hsiao1,2,3.
of Hematology and Oncology, Department of Internal Medicine, Kaohsiung
Medical University Hospital, Kaohsiung, Taiwan.
2 Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
3 Center for Liquid Biopsy, Kaohsiung Medical University, Kaohsiung, Taiwan.
4 University of Rutgers-Camden, New Jersey, USA.
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To the Editor,
Materials and Methods
|Figure 1. Figure 1A. Schematic view of CALR gene and the HRM primer sets at exon 9. Figure 1B The curve patterns of CALR exon 9 by the HRM method. a. The melting peaks of 1 and 2 showed CALR exon 9 del 1092-1143 (type 1) and 1151 ins TTGTC (type 2). b. CALR exon 9 del 1102-1137 ins CA, del 1099-1150, and del 1122-1140 were showed in HRM melting cures of 3, 4, and 5. c. The complicated mutations of 1151 ins TTGTC + 1142A>C, del 1099-1151 +1215G>T, and del 1114-1144+del 1221 in CALR exon 9 gene showed in the melting peaks of 6, 7, and 8. The wild type of CALR exon 9 was the horizontal line in the figures a, b, and c.|
|Table 1. CALR* mutation types and frequencies in 21 ET# patients.|
|Table 2. Characteristics of patients with different mutations.|