TY - JOUR AU - Ljungkvist, Marcus PY - 2017/11/01 Y2 - 2024/03/28 TI - Correlation to FVIII:C in two thrombin generation tests: TGA-CAT and INNOVANCE ETP JF - Mediterranean Journal of Hematology and Infectious Diseases JA - Mediterr J Hematol Infect Dis VL - 9 IS - 1 SE - Original Articles DO - 10.4084/mjhid.2017.064 UR - https://www.mjhid.org/mjhid/article/view/2017.064 SP - e2017064 AB - <p><strong>            Abstract</strong></p><p><strong>Background and objectives</strong></p><p>Several thrombin generation tests are available but few have been directly compared. Our primary aim was to investigate the correlation of two thrombin generation tests, thrombin generation assay-calibrated automated thrombogram (TGA-CAT) and INNOVANCE ETP, to factor VIII levels (FVIII:C) in a group of patients with hemophilia A.</p><p>The secondary aim was to investigate inter-laboratory variation for the TGA-CAT method.</p><p><strong>Methods</strong></p><p>Blood samples were taken from 45 patients with mild, moderate and severe hemophilia A. The TGA-CAT method was performed at both centers while the INNOVANCE ETP was only performed at the Stockholm center. Correlation between parameters was evaluated using Spearman´s rank correlation test. For determination of the TGA-CAT inter-laboratory variability Bland-Altman plots was used.</p><p><strong>Results</strong></p><p>The correlation for the INNOVANCE ETP and TGA-CAT methods with FVIII:C in persons with hemophilia (PWH) was r=0,701 and r=0,734 respectively.</p><p>The correlation between the two methods was r=0,546.</p><p>When dividing the study material into disease severity groups (mild, moderate and severe) based on FVIII levels, both methods fail to discriminate between them.</p><p>The variability of the TGA-CAT results performed at the two centers was reduced after normalization, before normalization 29% of values showed less than ±10% difference while after normalization the number increased to 41%.</p><p><strong>Conclusions</strong></p><p>Both methods correlate in an equal manner to FVIII:C in PWH but show a poor correlation to each other. The level of agreement for the TGA-CAT method was poor though slightly improved after normalization of data. Further improvement of standardization of these methods is warranted.</p> ER -