Nóbrega Litvoc

There is limited data regarding tuberculosis in hematopoietic stem cell transplantation (HSCT), especially in endemic countries. We conducted a retrospective cohort study including all patients who underwent matched unrelated allo-HSCT from 2007-2016 in the state of São Paulo, Brazil. Tuberculosis in this population was identified by record linkage between the national HSCT database (REREME) and the São Paulo State Tuberculosis Control Program database. Five tuberculosis cases (3 males) were identified and described among 1,223 allo-HSCT. The mean age was 25, and the mean time between HSCT and Tuberculosis diagnosis was 376 days. Density incidence rate and standardized incidence ratios (SIR) were compared between matched unrelated donor allo-HSCT and the São Paulo state Tuberculosis database (172,925 cases). Considering 2,718 patients/year, the density incidence rate in the allo-HSCT population was 183 cases/100.000 patients/year (SIR:4.9 and 95% CI 1.8-11:), much higher than the average rate for the whole population in the state of São Paulo for the same period: 38.8/100.000 inhabitants. In our study, allo-HSCT patients are highly vulnerable to tuberculosis disease, almost five times higher than in the general population.
Tuberculosis incidence in hematopoietic stem cell transplant (HSCT) recipients seems to be related to the prevalence in the country.[1,2] In HSCT patients, the risk of tuberculosis is higher in allogeneic stem cell transplantation (allo-HSCT). Tuberculosis incidence among allo-HSCT has been reported from 0.014% to 16.0%.[3] Data regarding Tuberculosis among HSCT is scarce even in endemic countries such as Brazil, one of the 30 most high-burden tuberculosis countries in the world.[4]
We aimed to describe tuberculosis's density incidence rate and standard incidence ratios (SIR) in matched unrelated allo-HSCT.

This retrospective cohort study includes all matched unrelated allo-HSCT patients in the São Paulo state from 2007 to 2016. We collected data from the São Paulo State Tuberculosis Control Program and the national allo-HSCT database from REREME at the National Institute of Cancer (INCA.,2021).[5]
REREME collects data from all matched unrelated donors allo-HSCT in the country. Probabilistic record linkage compares similarities between databases applying mathematical functions. Data were reviewed to correct errors and to standardize the content of the key variables – name, surname, and date of birth. Those fields are recorded in a Soundex code through parsing, substring, and transforming the text into a phonetic code to make the linkage between misrecorded information.[6] Record pairs are formed to be compared and classified into true pairs, non-pairs, and doubtful pairs and then manually reviewed to accept or reject the cases.
Available clinical, demographic, and epidemiological data of patients with tuberculosis were compared to the entire allo-HSCT patient cohort. During the study period, fluoroquinolones were recommended as bacterial prophylaxis in Brazil during the neutropenia until the engraftment. Microbiologic and molecular tests to diagnose tuberculosis are available in all centers. However, the decision to perform bronchoalveolar lavage (BAL) was of the clinical physician of each Hospital.
In order to calculate the incidence density rate of tuberculosis, we consider the contribution time from each patient, from the transplant date until death or until December 31^st, 2016, the final follow-up date. Long-term follow-up was characterized by death (outcome) or tuberculosis diagnosis. No autopsy cases data were available.
The study was approved by the institutional review board of the Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo.
Statistical Analysis. Density incidence rate and standard incidence ratios (SIR) were calculated to compare the incidence of tuberculosis in matched unrelated donor allo-HSCT and the general population in São Paulo during the study period. Nominal and ordinal variables were described as frequency and percentage; quantitative data, in median and range. All calculations were performed with Epi-Info 7.2.

In the state of Sao Paulo, from 2007 until 2016, 1,223 matched unrelated donor allo-HSCTs were performed. Among those, 1172 were new transplants, 39 were second-time transplant recipients, and 12 patients did the first transplant before the beginning of the study. Most patients were males (713 cases-60%), and the mean age at the onset of allo-HSCT was 25 years old. Acute Lymphoblastic Leukemia was the most common diagnosis (359 patients), followed by Acute Myeloid Leukemia and Chronic Myeloid Leukemia (Table 1).

Table 1. Allo-HSCT recipients from REREME 2007-2016 in São Paulo state, Brazil.

After transplantation, 440 patients have not survived, with a death rate of 35.9% and an average survival time until the death of 182.7 days; 172,925 cases were identified in the São Paulo State Tuberculosis Control Program database during the study period. Record linkage found 60 record pairs from two databases. After manual checking, 23 tuberculosis cases were identified among allo-HSCT patients. Five tuberculosis cases were diagnosed after, and 17 cases were diagnosed before the HSCT (Table 1 and supplementary material). The period without TB of all HSCT patients ranged from 11 days to 9 years. Post-transplantation cases were diagnosed in four different hospitals (2 public and 2 private) in the state of São Paulo. All new cases were pulmonary forms of tuberculosis except one neurological manifestation. The mean days between HSCT and Tuberculosis diagnosis were 376 days (ranging from 61 days until 1048 days). Four patients were cured after treatment with four drugs (RIPE); one case abandoned the treatment (Table 2). The incidence rate was 183 cases per 100.000 patients/year, and the SIR(4,9-95%CI:1.8-11.0). None of the 17 patients with previously treated Tuberculosis before HSCT reactivated during the follow-up period. The mean age of patients with previous tuberculosis was 19.3 years old. The mean time of diagnosis of active tuberculosis was 439.2 days before HSCT. The RIPE regimen was the most used in 10 cases (58.0%). The indication for HSCT was: Acute myeloid leukemia (AML) 8 (47.0%), Combined immunodeficiency 3 (17.0%), unspecified neutrophil functional disorder 3 (17.0%), Unspecified immunodeficiency 1 (5.8%) and other combined deficiencies 01 (5.8%). The clinical form of tuberculosis was pulmonary in 7 cases (41.1%), disseminated in 4 cases (23.5%), lymph node in 2 cases (11.7%), unspecified in 2 cases (11.7%), bone in 1 case (11.7%) and "extrapulmonary", without other definitions, in 1 case (11.7%).cTn.

Table 2. Density incidence rate and SIR of new tuberculosis cases diagnosed after allo-HSCT in the state of São Paulo from 2007 to 2016.

Our study is the first attempt to improve epidemiological data about Tuberculosis in HSCT in Brazil. Incidence of Tuberculosis matched unrelated allo-HSCT (183 cases/100,000 patients/year) was 4.9 times higher than the average rate for the population in the state of São Paulo (38.8/100,000 inhabitants). A Spanish mathematical modeling study has estimated an incidence rate of 135.6 cases per 100,000 inhabitants after allo-HSCT.[2] A recent Brazilian study reported a cumulative tuberculosis incidence of 3% among allo-HSCT.[7] Other authors observed a higher Tuberculosis incidence rate compared with our study. In Korea, a retrospective study examined 845 matched unrelated allo-HSCT and reported a Tuberculosis incidence of 654.2 /100,000 patients/year.[8]
Our study has limitations as most cases were treated based on clinical and radiological findings. However, a relevant finding was the number of patients, 17 with previously treated tuberculosis, that underwent HSCT and did not reactivate during the follow-up period. Another limitation of the study was that the susceptible testing was not available. However, four of the five patients that developed TB after HSCT were cured after treatment with four drugs, and one case abandoned the treatment. Thus, there was probably no tuberculosis resistance among the cases.

High Tuberculosis Density Incidence Rate in Matched Unrelated Allogeneic Stem Cell Transplantation Recipients in the State of São Paulo, Brazi