Scientific Letters
Vol. 9 No. 1 (2017): Review, Original Articles, Case Reports
https://doi.org/10.4084/mjhid.2017.020

Impact of Donor-Specific anti-HLA antibodies and donor KIR characteristics in haploidentical HSCT for beta-Thalassemia

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Received: December 5, 2016
Accepted: January 23, 2017
Published: March 1, 2017
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Haploidentical HSCT, KIR receptors, anti-HLA antibodies
Hematology

Authors

Marco Andreani
m.andreani@fondazioneime.org
Laboratory of Immunogenetics and Transplant Biology; IME Foundation at Polyclinic of Tor Vergata; Rome, Italy;, Italy.
Manuela Testi
Laboratory of Immunogenetics and Transplant Biology; IME Foundation at Polyclinic of Tor Vergata; Rome, Italy;, Italy.
Pietro Sodani
International Center for Transplantation in Thalassemia and Sickle Cell Anemia, IME Foundation at Polyclinic of Tor Vergata, Rome, Italy, Italy.
Maria Troiano
Laboratory of Immunogenetics and Transplant Biology; IME Foundation at Polyclinic of Tor Vergata; Rome, Italy;, Italy.
Andrea Di Luzio
Laboratory of Immunogenetics and Transplant Biology; IME Foundation at Polyclinic of Tor Vergata; Rome, Italy;, Italy.
Giuseppe Testa
Laboratory of Immunogenetics and Transplant Biology; IME Foundation at Polyclinic of Tor Vergata; Rome, Italy;, Italy.
Michela Falco
Laboratory of Clinical and Experimental Immunology, GianninaGaslini Institute, Genoa, Italy, Italy.
Elvira Poggi
Laboratory of Tissue Typing and Transplant Immunology, National Council of Researches IFT Unit of Rome S. Camillo Hospital – Regional Transplant Center Lazio, Rome, Italy, Italy.
Javid Gaziev
International Center for Transplantation in Thalassemia and Sickle Cell Anemia, IME Foundation at Polyclinic of Tor Vergata, Rome, Italy, Italy.
Antonina Piazza
Laboratory of Tissue Typing and Transplant Immunology, National Council of Researches IFT Unit of Rome S. Camillo Hospital – Regional Transplant Center Lazio, Rome, Italy, Italy.
In the present study we investigated the role of donor specific HLA antibodies (DSA) and donor KIR repertoire characteristics in a group of 18 patients affected by haemoglobinopathies who underwent haploidentical T cell depleted transplantation. Among these patients, 8 rejected the transplant while 10 had complete donor chimerism (CC). Five out of 8 patients (62.5%) who rejected graft had anti-HLA antibodies in the sera collected before transplant, while only 1 patient out of 10 (10%) with CC showed their presence (p=0,042). Notably, of the 5 HLA antibodies positive patients who rejected the graft 3 had DSA (2 for class I and 1 for class I and II) while none of the patients with CC had DSA. Among the 8 patients that experienced graft failure 4 were transplanted with a donor characterized by the lack of NK alloreactivity and 5 with a donor with a B content value <2. Although we analyzed a small cohort of patients, our data indicated that the presence of anti-HLA antibodies in patient sera, but not donor KIR characteristics, correlates with graft failure thus suggesting that analysis of anti-HLA antibodies should be taken into account in haploidentical transplant setting.

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Scientific Letters

Supporting Agencies

Fondazione Berloni per la ricerca scientifica
Marco Andreani, Laboratory of Immunogenetics and Transplant Biology; IME Foundation at Polyclinic of Tor Vergata; Rome, Italy;

Marco Andreani  is the Director of the Laboratory of Immunogenetics and Transplant Biology of the IME Foundation at  the University Policlinic of Tor Vergata in Rome, since 2005. His major research interests include Immunogenetics, HLA typing and Immunological Tolerance

How to Cite



“Impact of Donor-Specific anti-HLA antibodies and donor KIR characteristics in haploidentical HSCT for beta-Thalassemia” (2017) Mediterranean Journal of Hematology and Infectious Diseases, 9(1), p. e2017020. doi:10.4084/mjhid.2017.020.