Original Articles
Vol. 15 No. 1 (2023): Review Articles, Original Article, Scientific Letter, Case Reports Letter to the Editor
MULTIPARAMETRIC FLOW CYTOMETRY REFINES RISK STRATIFICATION IN NEWLY DIAGNOSED MULTIPLE MYELOMA PATIENTS
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: May 17, 2023
Accepted: July 23, 2023
Accepted: July 23, 2023
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Multiple myeloma (MM) is a heterogeneous malignancy characterized by the proliferation of abnormal plasma cells in the bone marrow. Multiparametric flow cytometry (MFC) plays a role in the work-up of the disease in view of the aberrant expression of surface antigens. Our study aimed at describing the antigenic profile detected by MFC in a series of newly diagnosed MM patients to correlate the level of expression with other features of the disease. Between April 2018 and June 2022, 84 consecutive MM patients were studied at presentation. CD56 and CD117 were commonly detected, while CD45, CD28, CD20, CD19, CD13 and CD33 were less recurrent. CD20 expression was associated with the type of secretory MM (p=0.041) and with a higher disease burden (p=0.038). CD28 positivity correlated with a lower platelet count at baseline (p=0.005) and with a lower rate of complete response (p=0.038). Furthermore, CD28 positivity and a lower CD138 expression tended to associate with the high-risk chromosomal translocations t(14;16) and t(4;14). The results of this study indicate that in the diagnostic work-up of MM, MFC may help to identify different patient subsets and improve risk stratification. These observations need to be validated in larger series of patients with a longer follow-up.
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Ethics Approval
MTP and MSDP designed the research study; FF wrote the manuscript; MLM and SI processed patient samples; MZL and GL performed cytogenetics tests; MSDP performed flow-cytometric analysis; MTP; FF and GL visited patients; AP performed statistical analysis; MM corrected the manuscript; RF and AG discussed the results and critically revised the manuscript. All authors have read and agreed to the published version of the manuscript.Supporting Agencies
This work was supported by Associazione Italiana Ricerca sul Cancro (AIRC), Special 5x1000 Program Metastases (21198), Milan, Italy (RF).How to Cite
“MULTIPARAMETRIC FLOW CYTOMETRY REFINES RISK STRATIFICATION IN NEWLY DIAGNOSED MULTIPLE MYELOMA PATIENTS” (2023) Mediterranean Journal of Hematology and Infectious Diseases, 15(1), p. e2023047. doi:10.4084/MJHID.2023.047.
Copyright (c) 2023 Francesca Fazio, Gianfranco Lapietra, Maria Zaira Limongi, Stefania Intoppa, Maria Laura Milani, Alfonso Piciocchi, Maurizio Martelli, Anna Guarini, Robin Foà, Maria Stefania De Propris, Maria Teresa Petrucci
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