Transfusion associated peak in Hb HPLC chromatogram – a case report

Main Article Content

Sonal Jain
Jasmita Dass
Hara Prasad Pati
(*) Corresponding Author:


High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC.

Downloads month by month


Download data is not yet available.

Article Details

Author Biographies

Sonal Jain, All India Institute of Medical Sciences

MD, DNB, Department of Hematology

Jasmita Dass, All India Institute of Medical Sciences

MD, Department of Hematology

Hara Prasad Pati, All India Institute of Medical Sciences

MD, FISHTM, FIMSA, Department of Hematology