Abdoul Karim OUATTARA, Pouiré YAMEOGO, Birama DIARRA, Dorcas OBIRI-YEBOAH, Albert Theophane YONLI, Tegwindé Rebeca COMPAORE, Serge Théophile SOUBEIGA, Florencia Wenkuuni DJIGMA, Jacques Simpore
  • Abdoul Karim OUATTARA
    CERBA/LABIOGENE, Université de Ouagadougou 01 BP 364Ouagadougou 01, Burkina Faso,
  • Pouiré YAMEOGO
    CERBA/LABIOGENE, Université de Ouagadougou 01 BP 364Ouagadougou 01, Burkina Faso,
  • Birama DIARRA
    CERBA/LABIOGENE, Université de Ouagadougou 01 BP 364Ouagadougou 01, Burkina Faso,
    Affiliation not present
  • Albert Theophane YONLI
    CERBA/LABIOGENE, Université de Ouagadougou 01 BP 364Ouagadougou 01, Burkina Faso,
  • Tegwindé Rebeca COMPAORE
    CERBA/LABIOGENE, Université de Ouagadougou 01 BP 364Ouagadougou 01, Burkina Faso,
  • Serge Théophile SOUBEIGA
    CERBA/LABIOGENE, Université de Ouagadougou 01 BP 364Ouagadougou 01, Burkina Faso,
  • Florencia Wenkuuni DJIGMA
    CERBA/LABIOGENE, Université de Ouagadougou 01 BP 364Ouagadougou 01, Burkina Faso,


The G-6-PD deficiency has an important polymorphism with genotypic variants such as 202A/376G, 376G/542T and 376G/968T known in West African populations. It would confer protection against severe forms of malaria although there are differences between the various associations in different studies. In this study we genotyped six (06) variants of the G-6-PD gene in people with symptomatic malaria in urban areas in Burkina Faso.

One hundred and eighty-two (182) patients who tested positive using rapid detection test and microscopy were included in this study. A regular PCR with the GENESPARK G6PD African kit was run followed by electrophoresis, allowing initially to genotype six SNPs (G202A, A376G, A542T, G680T, C563T and T968C). Women carrying the mutations 202A and/or 376G were further typed by real-time PCR using TaqMan probes rs1050828 and rs1050829.

In the study population the G-6-PD deficiency prevalence was 9.9%. In addition of G-6-PD A- (202A/376G) variants, 376G/542Tand 376G/968T were detected. Hemoglobin electrophoresis revealed that 22.5% (41/182) of the individuals had HbAC compared with 2.2% with HbAS and one individual had double heterozygous HbSC. There was no correlation between the G-6-PD deficiency or haemoglobinopathies and symptomatic malaria in this study.

As opposed to previous genotyping studies carried out in Burkina Faso, this study shows for the first time the presence of the variant A- (376G/968C) and warrants further investigation at the national level and in specific ethnic groups.


Anemia, G-6-PD, Malaria, Parassitosis

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Submitted: 2016-04-11 21:22:37
Published: 2016-06-15 00:00:00
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Simpore J, Ilboudo D, Damintoti K, Sawadogo L, Maria E, Binet S, Nitiema H, Ouedraogo P, Pignatelli S, Nikiema JB. Glucose-6-phosphate dehydrogenase deficiency and sickle cell disease in Burkina Faso. Pak J Biol Sci 2007, 10:409-414.

Hedrick PW. Population genetics of malaria resistance in humans. Heredity (Edinb) 2011, 107:283-304.

Modiano D, Bancone G, Ciminelli BM, Pompei F, Blot I, Simpore J, Modiano G. Haemoglobin S and haemoglobin C: 'quick but costly' versus 'slow but gratis' genetic adaptations to Plasmodium falciparum malaria. Hum Mol Genet 2008, 17:789-799.

Modiano D, Luoni G, Sirima BS, Simpore J, Verra F, Konate A, Rastrelli E, Olivieri A, Calissano C, Paganotti GM, D'Urbano L, Sanou I, Sawadogo A, Modiano G, Coluzzi M. Haemoglobin C protects against clinical Plasmodium falciparum malaria. Nature 2001, 414:305-308.

Mangano VD, Kabore Y, Bougouma EC, Verra F, Sepulveda N, Bisseye C, Santolamazza F, Avellino P, Tiono AB, Diarra A, Nebie I, Rockett KA, Sirima SB, Modiano D, Malaria G. E. N. Consortium. Novel Insights Into the Protective Role of Hemoglobin S and C Against Plasmodium falciparum Parasitemia. J Infect Dis 2015, 212:626-634.

Luzzatto L. Sickle cell anaemia and malaria. Mediterr J Hematol Infect Dis 2012, 4:e2012065.

Howes RE, Dewi M, Piel FB, Monteiro WM, Battle KE, Messina JP, Sakuntabhai A, Satyagraha AW, Williams TN, Baird JK, Hay SI. Spatial distribution of G6PD deficiency variants across malaria-endemic regions. Malar J 2013, 12:418.

Cappellini MD, Fiorelli G. Glucose-6-phosphate dehydrogenase deficiency. The Lancet, 371:64-74.

Minucci A, Moradkhani K, Hwang MJ, Zuppi C, Giardina B, Capoluongo E. Glucose-6-phosphate dehydrogenase (G6PD) mutations database: review of the "old" and update of the new mutations. Blood Cells Mol Dis 2012, 48:154-165.

Luzzatto L, Seneca E. G6PD deficiency: a classic example of pharmacogenetics with on-going clinical implications. Br J Haematol 2014, 164:469-480.

Ouattara AK, Bisseye C, Bazie BV, Diarra B, Compaore TR, Djigma F, Pietra V, Moret R, Simpore J. Glucose-6-phosphate dehydrogenase (G6PD) deficiency is associated with asymptomatic malaria in a rural community in Burkina Faso. Asian Pac J Trop Biomed 2014, 4:655-658.

De Araujo C, Migot-Nabias F, Guitard J, Pelleau S, Vulliamy T, Ducrocq R. The role of the G6PD AEth376G/968C allele in glucose-6-phosphate dehydrogenase deficiency in the seerer population of Senegal. Haematologica 2006, 91:262-263.

Clark TG, Fry AE, Auburn S, Campino S, Diakite M, Green A, Richardson A, Teo YY, Small K, Wilson J, Jallow M, Sisay-Joof F, Pinder M, Sabeti P, Kwiatkowski DP, Rockett KA. Allelic heterogeneity of G6PD deficiency in West Africa and severe malaria susceptibility. Eur J Hum Genet 2009, 17:1080-1085.

Maiga B, Dolo A, Campino S, Sepulveda N, Corran P, Rockett KA, Troye-Blomberg M, Doumbo OK, Clark TG. Glucose-6-phosphate dehydrogenase polymorphisms and susceptibility to mild malaria in Dogon and Fulani, Mali. Malar J 2014, 13:270.

Carter N, Pamba A, Duparc S, Waitumbi JN. Frequency of glucose-6-phosphate dehydrogenase deficiency in malaria patients from six African countries enrolled in two randomized anti-malarial clinical trials. Malar J 2011, 10:241.

Modiano D, Luoni G, Sirima BS, Lanfrancotti A, Petrarca V, Cruciani F, Simpore J, Ciminelli BM, Foglietta E, Grisanti P, Bianco I, Modiano G, Coluzzi M. The lower susceptibility to Plasmodium falciparum malaria of Fulani of Burkina Faso (west Africa) is associated with low frequencies of classic malaria-resistance genes. Trans R Soc Trop Med Hyg 2001, 95:149-152.

Kafando E, Sawadogo M, Cotton F, Vertongen F, Gulbis B. Neonatal screening for sickle cell disorders in Ouagadougou, Burkina Faso: a pilot study. J Med Screen 2005, 12:112-114.

Amoako N, Asante KP, Adjei G, Awandare GA, Bimi L, Owusu-Agyei S. Associations between Red Cell Polymorphisms and Plasmodium falciparum Infection in the Middle Belt of Ghana. PLoS ONE 2014, 9:e112868.

Travassos MA, Coulibaly D, Laurens MB, Dembele A, Tolo Y, Kone AK, Traore K, Niangaly A, Guindo A, Wu Y, Berry AA, Jacob CG, Takala-Harrison S, Adams M, Shrestha B, Mu AZ, Kouriba B, Lyke KE, Diallo DA, Doumbo OK, Plowe CV, Thera MA. Hemoglobin C Trait Provides Protection From Clinical Falciparum Malaria in Malian Children. J Infect Dis 2015, 212:1778-1786.

Malaria Genomic Epidemiology Network. Reappraisal of known malaria resistance loci in a large multicenter study. Nat Genet 2014, 46:1197-1204.

Shah SS, Rockett KA, Jallow M, Sisay-Joof F, Bojang KA, Pinder M, Jeffreys A, Craik R, Hubbart C, Wellems TE, Kwiatkowski DP, Malaria G. E. N. Consortium. Heterogeneous alleles comprising G6PD deficiency trait in West Africa exert contrasting effects on two major clinical presentations of severe malaria. Malar J 2016, 15:13.

Mombo LE, Ntoumi F, Bisseye C, Ossari S, Lu CY, Nagel RL, Krishnamoorthy R. Human genetic polymorphisms and asymptomatic Plasmodium falciparum malaria in Gabonese schoolchildren. Am J Trop Med Hyg 2003, 68:186-190.

Manjurano A, Sepulveda N, Nadjm B, Mtove G, Wangai H, Maxwell C, Olomi R, Reyburn H, Riley EM, Drakeley CJ, Clark TG, Malaria G. E. N. Consortium. African glucose-6-phosphate dehydrogenase alleles associated with protection from severe malaria in heterozygous females in Tanzania. PLoS Genet 2015, 11:e1004960.

Sirugo G, Predazzi IM, Bartlett J, Tacconelli A, Walther M, Williams SM. G6PD A- deficiency and severe malaria in The Gambia: heterozygote advantage and possible homozygote disadvantage. Am J Trop Med Hyg 2014, 90:856-859.

Igbeneghu C, Olisekodiaka MJ, Akinola FFS, Odaibo AB. Impact of Haemoglobin Variants AS and AC on Asymptomatic Falciparum Malaria among Adults in Iwo, Southwestern Nigeria. Scholars Journal of Applied Medical Sciences 2015, 3:17-20.

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