Haptoglobin phenotypes and susceptibility of Schistosoma parasites infection in central Sudan

Ashraf Siddig Yousif, Atif Abdelrahman Elagib
  • Atif Abdelrahman Elagib
    National Centre for Research, Ministry of Higher Education and Scientific Research, Sudan


Haptoglobin (Hp) is an acute phase protein that binds the free haemoglobin (Hb), thus preventing iron loss and renal damage. Hp also has anti-oxidative and immunomodulatory properties. Three Hp phenotypes have been identified in human: Hp1-1, Hp2-1 and Hp2-2. Hp polymorphisms have been related to susceptibility of various diseases. In this study we aimed to assess the possible association of Hp phenotypes polymorphism to Schistosoma parasites infection in central Sudan. We have investigated the Hp phenotypes polymorphism distribution in the serum of 125 (93 S. mansonai, 13 S. haematobium and 19 infected with both ‘’co-infection’’) parasitologically confirmed infected individuals and 208 healthy individuals served as control. Hp phenotypes have been determined by polyacrylamide gel electrophoresis followed by benzidine staining. Our study revealed that Hp1-1 percentage frequency was significantly higher in infected individuals than healthy control individuals 51% and 26% respectively. Our data suggest that Hp1-1 phenotype may upsurge the susceptibility to Schistosoma parasites infection in central Sudan.


: Haptoglobin, Polymorphism, Schistosoma, Infection and Susceptibility

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Submitted: 2017-03-26 01:51:21
Published: 2017-07-01 00:00:00
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Alayash AI. Haptoglobin: Old protein with new functions. Vol. 412, Clinica Chimica Acta. 2011. p. 493–8. http://www.sciencedirect.com/science/article/pii/S0009898110007576

Fagoonee S, Gburek J, Hirsch E, Marro S, Moestrup SK, Laurberg JM, et al. Plasma protein haptoglobin modulates renal iron loading. Am J Pathol [Internet]. 2005;166(4):973–83. Available from: http://dx.doi.org/10.1016/S0002-9440(10)62319-X


Langlois MR, Delanghe JR. Biological and clinical significance of haptoglobin polymorphism in humans. Vol. 42, Clinical Chemistry. 1996. p. 1589–600. https://www.ncbi.nlm.nih.gov/pubmed/8855140

Speeckaert R, Brochez L, Lambert J, van Geel N, Speeckaert MM, Claeys L, et al. The haptoglobin phenotype influences the risk of cutaneous squamous cell carcinoma in kidney transplant patients. J Eur Acad Dermatol Venereol [Internet]. 2012;26(5):566–71. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21575065

Kasvosve I, Speeckaert MM, Speeckaert R, Masukume G, Delanghe JR. Haptoglobin polymorphism and infection. Vol. 50, Advances in clinical chemistry. 2010. p. 23–46. http://www.sciencedirect.com/science/article/pii/S0065242310500027

Mahmud SM, Koushik A, Duarte-Franco E, Costa J, Fontes G, Bicho M, et al. Haptoglobin phenotype and risk of cervical neoplasia: A case-control study. Clin Chim Acta. 2007;385(1–2):67–72. http://www.sciencedirect.com/science/article/pii/S000989810700366X

Napolioni V, Giannì P, Carpi FM, Concetti F, Lucarini N. Haptoglobin (HP) polymorphisms and human longevity: A cross-sectional association study in a Central Italy population. Clin Chim Acta. 2011;412(7–8):574–7. https://www.ncbi.nlm.nih.gov/pubmed/21147083

Deganello, R., Cruciani, M., Beltramello, C., Duncan, O., Oyugi, V. and Montresor A. Schistosoma haematobium and Schistosoma mansoni among children, Southern Sudan. Emerg Infect Dis. 2007;13:1–5. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2851532/

Elhag SM, Abdelkareem EA, Yousif AS, Frah EA, Mohamed AB. Detection of schistosomiasis antibodies in urine patients as a promising diagnostic maker. Asian Pac J Trop Med. 2011;4(10):773–7. https://www.ncbi.nlm.nih.gov/pubmed/22014731

Sleigh A, Xueming Li, Jackson S, Huang K. Eradication of schistosomiasis in Guangxi, China. Part 3. Community diagnosis of the worst-affected areas and maintenance strategies for the future. Bull World Health Organ. 1998;76(6):581–90. https://www.ncbi.nlm.nih.gov/pubmed/10191554

Ibrahim N, Baleela R. Association of Hp 1-1 with liver disorders among Sudanese patients. Am J Sci Ind Res [Internet]. 2012;3(6):403–5. Available from: http://www.scihub.org/AJSIR

Rania M. H. Baleela; Nada E. Ibrahim; Omran F. Osman and Atif A. Elagib2. Distribution of Haptoglobin Phenotypes among Patients with HIV/AIDS, Hepatitis B, Liver Cirrhosis and Chronic Renal Failure in Sudan. Sudan J Sci. 2012;5(1):1–6. http://khartoumspace.uofk.edu/handle/123456789/22372

Elagib AA, Kider AO, Åkerström B, Elbashir MI. Association of the haptoglobin phenotype (1-1) with falciparum malaria in Sudan. Trans R Soc Trop Med Hyg. 1998;92(3):309–11. https://www.ncbi.nlm.nih.gov/pubmed/9861405

Yousif AS, Abdelkareem E a., Elhag SM, Elgimeaabi L a., Ahmed M a., Frah E a., et al. Circulating antigens of Schistosoma parasites in urine of schistosomiasis patients in Central Sudan. J Infect Dis Immun [Internet]. 2009;1(2):011–5. Available from: http://www.academicjournals.org/journal/JIDI/article-abstract/0F9FF8C4263

Davis. I. and Orenstein. J. DISC electrophporesis, Acrylamide gel columns. Vol. 2, Methods in immunology and immunochemistry. 1968. 38-47 p.

Linke RP. Typing and subtyping of haptoglobin from native serum using disc gel electrophoresis in alkaline buffer: Application to routine screening. Anal Biochem. 1984;141(1):55–61. https://www.ncbi.nlm.nih.gov/pubmed/6496936

Chapman SJ, Hill A V. Human genetic susceptibility to infectious disease. Nat Rev Genet [Internet]. 2012;13(3):175–88. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22310894

Brouwer MC, de Gans J, Heckenberg SG, Zwinderman AH, van der Poll T, van de Beek D. Host genetic susceptibility to pneumococcal and meningococcal disease: a systematic review and meta-analysis. Vol. 9, The Lancet Infectious Diseases. 2009. p. 31–44. https://www.ncbi.nlm.nih.gov/pubmed/19036641

Speeckaert R, Colebunders B, Boelaert JR, Brochez L, Van Acker J, Van Wanzeele F, et al. Association of haptoglobin phenotypes with the development of Kaposi’s sarcoma in HIV patients. Arch Dermatol Res. 2011;303:763–9. http://link.springer.com/article/10.1007/s00403-011-1161-9

Vitalis Z, Altorjay I, Tornai I, Palatka K, Kacska S, Palyu E, et al. Phenotypic polymorphism of haptoglobin: A novel risk factor for the development of infection in liver cirrhosis. Hum Immunol. 2011;72(4):348–54. https://www.ncbi.nlm.nih.gov/pubmed/21262313

Mundaray Fernández N, Fernández-Mestre M. The Role of haptoglobin genotypes in Chagas disease. Dis Markers. 2014;2014:1–6. https://www.hindawi.com/journals/dm/2014/793646/

Perdijk O, Arama C, Giusti P, Maiga B, Troye-Blomberg M, Dolo A, et al. Haptoglobin phenotype prevalence and cytokine profiles during Plasmodium falciparum infection in Dogon and Fulani ethnic groups living in Mali. Malar J [Internet]. 2013;12(1):432. Available from: http://www.malariajournal.com/content/12/1/432

Quaye IKE, Ekuban FA, Goka BQ, Adabayeri V, Kurtzhals JAL, Gyan B, et al. Haptoglobin 1-1 is associated with susceptibility to severe Plasmodium falciparum malaria. Trans R Soc Trop Med Hyg [Internet]. 2000;94(2):216–9. Available from: http://trstmh.oxfordjournals.org.ezp.lib.unimelb.edu.au/content/94/2/216

Tseng CF, Lin CC, Huang HY, Liu HC, Mao SJT. Antioxidant role of human haptoglobin. In: Proteomics. 2004. p. 2221–8. https://www.ncbi.nlm.nih.gov/pubmed/15274115

Cook RM, Carvalho-Queiroz C, Wilding G, LoVerde PT. Nucleic acid vaccination with Schistosoma mansoni antioxidant enzyme cytosolic superoxide dismutase and the structural protein filamin confers protection against the adult worm stage. Infect Immun. 2004;72(10):6112–24. https://www.ncbi.nlm.nih.gov/pubmed/15385516

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