EBV AND HHV-6 CIRCULATING SUBTYPES IN PEOPLE LIVING WITH HIV IN BURKINA FASO, IMPACT ON CD4 T CELL COUNT AND HIV VIRAL LOAD

Lassina TRAORE, Ouéogo NIKIEMA, Abdoul Karim OUATTARA, Tegwindé Rébéca COMPAORE, Serge Théophile SOUBEIGA, Birama DIARRA, Dorcas OBIRI-YEBOAH, Pegdwendé Abel SORGHO, Florencia Wendkuuni DJIGMA, Cyrille BISSEYE, Albert Théophane YONLI, Jacques SIMPORE
  • Lassina TRAORE
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Burkina Faso | ttl.lass@yahoo.fr
  • Ouéogo NIKIEMA
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Burkina Faso
  • Abdoul Karim OUATTARA
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Burkina Faso
  • Tegwindé Rébéca COMPAORE
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Burkina Faso
  • Serge Théophile SOUBEIGA
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Burkina Faso
  • Birama DIARRA
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Mali
  • Dorcas OBIRI-YEBOAH
    Deparment of Microbiology and Immunolpgy, School of Medical Sciences, University of Cape Coast, Ghana, Ghana
  • Pegdwendé Abel SORGHO
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Burkina Faso
  • Florencia Wendkuuni DJIGMA
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Burkina Faso
  • Cyrille BISSEYE
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Gabon
  • Albert Théophane YONLI
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Burkina Faso
  • Jacques SIMPORE
    Biomolecular Research Center Pietro Annigoni (CERBA); LABIOGENE UFR/SVT, University Ouaga I Pr Joseph KI-ZERBO, Burkina Faso

Abstract

Epstein Barr Virus (EBV) and Human Herpes Virus 6 (HHV-6) are responsible for severe diseases, particularly in immunocompromised persons. There are poor data on the infection with these opportunistic viruses in Burkina Faso.

The purpose of this study is to characterize EBV and HHV-6 subtypes and to assess their impact on CD4 T cell count, HIV-1 viral load and antiretroviral treatment in people living with HIV-1.

The study population consisted of 238 HIV-positive patients with information on CD4 count, HIV-1 viral load and HAART. Venous blood samples collected on EDTA tubes were used for EBV and HHV-6 Real Time PCR subtyping.

An infection rate of 6.7% (16/238) and 7.1% (17/238) were found respectively for EBV and HHV-6 in the present study. Among EBV infections, similar prevalences were noted for both subtypes (3.9% [9/238] for EBV-1 vs 4.6% [11/238] for EBV-2) with 2.1% (5/238) of co-infection. HHV-6A infection represented 6.3% (15/238) of the study population against 5.0% (12/238) for HHV-6B. . EBV-2 infection was significantly higher in patients with CD4 count ≥ 500 compared to those with CD4 count less than 500 cells (1.65% vs 8.56%, p = 0,011). The prevalence of EBV and HHV-6 infections were almost similar in HAART-naive and HAART-experienced patients.

The present study provides information on the prevalence of EBV and HHV-6 subtypes in people living with HIV-1 in Burkina Faso. The study also suggests that HAART treatment has no effect on infection with these opportunistic viruses in people living with HIV-1.

Keywords

EBV, HHV-6, HIV-1, subtype, CD4 T cell count, viral load and treatment

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Published: 2017-09-01 00:00:00
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