Transfusion associated peak in Hb HPLC chromatogram – a case report

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Sonal Jain
Jasmita Dass *
Hara Prasad Pati
(*) Corresponding Author:
Jasmita Dass | drjasmita@gmail.com

Abstract

High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC.


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Author Biographies

Sonal Jain, All India Institute of Medical Sciences

MD, DNB, Department of Hematology

Jasmita Dass, All India Institute of Medical Sciences

MD, Department of Hematology

Hara Prasad Pati, All India Institute of Medical Sciences

MD, FISHTM, FIMSA, Department of Hematology