Cytomorphology of Chimeric Antigen Receptor T-Cells (CAR-T)
Main Article Content
Keywords
Chimeric antigen receptor T cells, CAR-T, cytomorphology, immunotherapy, cell therapy
Abstract
Chimeric antigen receptor (CAR) T cells represent one of the newest frontiers of cell therapy. Their application currently involves relapsed/refractory aggressive B cell lymphoma and leukemia as a standard of care, while several studies are exploring CAR-T to treat multiple myeloma and other hematological malignancies. Here we describe the cytomorphology of CAR-T collected from the leftovers of infusion bags, and therefore before the encounter with the antigen, with, among others, a peculiar population of giant lymphoid cells with blastoid features and hypertrophic centrosome.
Downloads
References
2. Kupfer A, Swain SL, Janeway CA, Singer SJ. The specific direct interaction of helper T cells and antigen-presenting B cells (microtubule organizing center/Golgi apparatus/immunofluorescence). Vol. 83, Immunology. 1986.
3. Hui KL, Upadhyaya A. Dynamic microtubules regulate cellular contractility during T-cell activation. Proc Natl Acad Sci U S A 2017 May 23;114(21):E4175–83
4. Brooks S, Frey N, Porter D, June C, Lacey S, Bagg A. The cytological features of CAR(T) cells. Vol. 175, British Journal of Haematology. Blackwell Publishing Ltd; 2016. p. 366.
5. Han X, Wang C, Zhou J. Chimeric antigen receptor T (CAR-T) cells present with reactive and pleomorphic morphology in bone marrow. Vol. 94, American Journal of Hematology. Wiley-Liss Inc.; 2019. p. 1297–8.
6. Faude S, Wei J, Muralidharan K, et al. Absolute lymphocyte count proliferation kinetics after CAR T-cell infusion impact response and relapse. Blood Adv. 2021 Apr 27;5(8):2128–36.
Article Sidebar
Article Details
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Transfer of Copyright and Permission to Reproduce Parts of Published Papers. Authors will grant copyright of their articles to the Institute of Hematology, Catholic University, Rome . No formal permission will be required to reproduce parts (tables or illustrations) of published papers, provided the source is quoted appropriately and reproduction has no commercial intent. Reproductions with commercial intent will require written permission and payment of royalties.