Original Articles
Vol. 13 No. 1 (2021): Mediterranean Journal of Hematology and Infectious Diseases, Volume 13, 2021
CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIA
In vitro Malaria cultures
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Received: February 11, 2021
Accepted: April 11, 2021
Accepted: April 11, 2021
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- 2021-04-29 (1)
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Background: Malaria morbidity and mortality, almost entirely from Plasmodium falciparum, are still rampant in Africa: therefore, it is important to study the biology of the parasite and the parasite-host cell interactions. In vitro cultivation of Plasmodium falciparum is most useful for this purpose, as well as for investigating drug resistance and possible new therapies. Here we report that the Trager & Jensen continuous culture of P. falciparum can be established in a laboratory in Tanzania with minimal facilities and with modest expenditure.
Methodology: This was an in-vitro set up of continuous culture of Plasmodium falciparum study, carried out in 2016-2020 at Muhimbili university of health and allied sciences, Dar-es salaam. Parasite samples were obtained from patients with acute malaria, frozen parasites, and live cultures. Data was collected and analyzed using GraphPad Prism version 8.
Results: We have successfully achieved exponential growth of existing strains that are used worldwide, as well as of parasites in clinical samples from patients with acute malaria. In the aim to optimize growth we have compared human serum and bovine serum albumin as components of the culture media. Additionally, culture synchronization has been achieved using sorbitol.
Conclusion: This experimental system is now available to our institution and to researchers aiming at investigating drug sensitivity and mechanisms of protection against Plasmodium falciparum that accrue from various genes expressed in red cells.
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Citations
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Supporting Agencies
MUHAS-SIDA Small grants, MMV, MAGI, Julie Makani has a grant from NIHHow to Cite
“CONTINUOUS CULTURES OF PLASMODIUM FALCIPARUM ESTABLISHED IN TANZANIA FROM PATIENTS WITH ACUTE MALARIA : In vitro Malaria cultures ” (2021) Mediterranean Journal of Hematology and Infectious Diseases, 13(1), p. Page e2021036. doi:10.4084/mjhid.2021.036.
Copyright (c) 2021 Florence Urio, Matilda Mkombachepa, Gration Rwegasira, Twilumba Makene, Billy Ngasala, Teddy Mselle, Julie Makani, Lucio Luzzatto
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
