Cytomorphology of Chimeric Antigen Receptor T-Cells (CAR-T)

Main Article Content

Eugenio Galli http://orcid.org/0000-0002-2839-916X
Silvia Bellesi
Marcello Viscovo
Federica Sorà
Stefan Hohaus
Nicola Piccirillo
Luca Laurenti
Patrizia Chiusolo
Valerio De Stefano
Simona Sica
Gina Zini

Keywords

Chimeric antigen receptor T cells, CAR-T, cytomorphology, immunotherapy, cell therapy

Abstract

Chimeric antigen receptor (CAR) T cells represent one of the newest frontiers of cell therapy. Their application currently involves relapsed/refractory aggressive B cell lymphoma and leukemia as a standard of care, while several studies are exploring CAR-T to treat multiple myeloma and other hematological malignancies. Here we describe the cytomorphology of CAR-T collected from the leftovers of infusion bags, and therefore before the encounter with the antigen, with, among others, a peculiar population of giant lymphoid cells with blastoid features and hypertrophic centrosome.

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References

1. Magnani CF, Gaipa G, Lussana F, et al. Sleeping Beauty–engineered CAR T cells achieve antileukemic activity without severe toxicities. J Clin Invest . 2020 Nov 2 130(11):6021–33.
2. Kupfer A, Swain SL, Janeway CA, Singer SJ. The specific direct interaction of helper T cells and antigen-presenting B cells (microtubule organizing center/Golgi apparatus/immunofluorescence). Vol. 83, Immunology. 1986.
3. Hui KL, Upadhyaya A. Dynamic microtubules regulate cellular contractility during T-cell activation. Proc Natl Acad Sci U S A 2017 May 23;114(21):E4175–83
4. Brooks S, Frey N, Porter D, June C, Lacey S, Bagg A. The cytological features of CAR(T) cells. Vol. 175, British Journal of Haematology. Blackwell Publishing Ltd; 2016. p. 366.
5. Han X, Wang C, Zhou J. Chimeric antigen receptor T (CAR-T) cells present with reactive and pleomorphic morphology in bone marrow. Vol. 94, American Journal of Hematology. Wiley-Liss Inc.; 2019. p. 1297–8.
6. Faude S, Wei J, Muralidharan K, et al. Absolute lymphocyte count proliferation kinetics after CAR T-cell infusion impact response and relapse. Blood Adv. 2021 Apr 27;5(8):2128–36.