COMPARISON OF A DNA BASED PCR APPROACH WITH CONVENTIONAL METHODS FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN MOROCCO

Fathiah Zakham, Oufae Lahlou, Mohammed Akrim, Nada Bouklata, Sanae Jaouhari, Khalid Sadki, Fouad Seghrouchni, Mohammed Elmzibri, Abdelaziz Benjouad, Moulay Mustapha Ennaji, Rajae Elaouad
  • Fathiah Zakham
    Affiliation not present
  • Oufae Lahlou
    Affiliation not present
  • Mohammed Akrim
    Affiliation not present
  • Nada Bouklata
    Affiliation not present
  • Sanae Jaouhari
    Affiliation not present
  • Khalid Sadki
    Affiliation not present
  • Fouad Seghrouchni
    Affiliation not present
  • Mohammed Elmzibri
    Affiliation not present
  • Abdelaziz Benjouad
    Affiliation not present
  • Moulay Mustapha Ennaji
    University Hassan II. Mohammedia_Casablanca Faculty of Science and Techniques. Laboratory of Virology, Hygiene & Microbiology, Morocco | m.ennaji@yahoo.fr
  • Rajae Elaouad
    Affiliation not present

Abstract

Background: Worldwide, tuberculosis (TB) is a major public health problem and the rapid diagnosis and appropriate chemotherapy become the first priority and a serious challenge to improve TB treatment.

In the objective of early TB diagnosis and rapid detection of Mycobacterium tuberculosis (MTB) in the clinical specimens, the utility of the Polymerase Chain Reaction (PCR) using the Insertion Sequence 6110 (IS6110) as target was compared to conventional methods.

Methods: Out of 305 patients with different clinical manifestations: suspected, new, drug relapse, drug failure and chronic cases were enrolled in this study and tested by mycobacteriological and PCR techniques for the investigation about the tubercle bacilli.

Results: The results of the in house IS6110 PCR showed a good sensitivity (92, 42%) and high specificity (98%), the positive and negative predictive values were 96.4 % and 95.3 % respectively.

Conclusion: This study showed clearly that the PCR testing using the IS6110 in the routine analysis is a potential tool for the rapid TB diagnosis, especially for critical cases and would be of great interest to help the clinician in the misdiagnosed critical cases by the traditional radiology.

Keywords

Tuberculosis; Mycobacterium tuberculosis; PCR,;IS6110

Full Text:

PDF
HTML
Submitted: 2014-06-13 09:31:48
Published: 2012-08-10 00:00:00
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
131

Views:
PDF
124
HTML
688

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2016 Mediterranean Journal of Hematology and Infectious Diseases

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

 

The Mediterranean Journal of Hematology and Infectious Diseases [eISSN 2035-3006] is owned by the U.C.S.C. and it is published by PAGEPress®, Pavia, Italy. All credits and honors to PKP for their OJS.
 
 
© PAGEPress 2008-2017     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185